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Table. 4 Linear regression analysisa results of species-specific nucleotide position of ITS2 gene

From: Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Locus Species distinction Nucleotide Nb R2 Sest c f(x)
rI.370G An.kleini G 9 1.000 0.65 0.998x – 0.547
rI.372T An.kleini T 9 0.993 3.29 1.029x − 5.815
rI.380T An.kleini T 9 0.968 6.83 1.012x + 7.43
rI.377T An.sinensis T 9 0.894 12.4 0.933x + 10.0
rI.378G An.belenrae G 9 0.996 2.28 1.003x + 0.071
rI.389T An.belenrae T 9 0.999 1.33 0.996x + 1.038
rI.400A An.belenrae A 9 0.865 14.0 1.042x -3.667
  1. aLinear regression analyses for 8 nucleotide positions were done by GraphPad Prism. The selected markers and corresponding equations for species distinction are underlined
  2. bThe number of x values used for regression analysis
  3. cStandard error of estimate