Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Table. 4 Linear regression analysis^a results of species-specific nucleotide position of ITS2 gene

Locus	Species distinction	Nucleotide	N^b	R²	S_est ^c	f(x)
rI.370G	An.kleini	G	9	1.000	0.65	0.998x – 0.547
rI.372T	An.kleini	T	9	0.993	3.29	1.029x − 5.815
rI.380T	An.kleini	T	9	0.968	6.83	1.012x + 7.43
rI.377T	An.sinensis	T	9	0.894	12.4	0.933x + 10.0
rI.378G	An.belenrae	G	9	0.996	2.28	1.003x + 0.071
rI.389T	An.belenrae	T	9	0.999	1.33	0.996x + 1.038
rI.400A	An.belenrae	A	9	0.865	14.0	1.042x -3.667

^aLinear regression analyses for 8 nucleotide positions were done by GraphPad Prism. The selected markers and corresponding equations for species distinction are underlined
^bThe number of x values used for regression analysis
^cStandard error of estimate

ISSN: 1475-2875