Table 5 Dilutions of clinical DBS containing P. falciparum measured by the simplified duplex assay
Dilution | Nominal concentration | Pf qRT-PCR | Pan qRT-PCR | ||||
|---|---|---|---|---|---|---|---|
Parasites/mL blood | Log10 copies/mL blood | Log10 copies/spot | Parasites/mL blood | Log10 copies/spot | Parasites/mL blood | Log10 copies/spot | |
Neat | 2.5 × 102 | 6.3 | 5.0 | 1.9 × 102 | 4.9 | 5.9 × 102 | 5.3 |
1:4 | 6.3 × 101 | 5.7 | 4.4 | 4.0 × 101 | 4.2 | 9.6 × 101 | 4.6 |
1:16 | 1.6 × 101 | 5.1 | 3.8 | 1.0 × 101 | 3.6 | 2.5 × 101 | 4.0 |
1:64 | 4 | 4.5 | 3.2 | 1.8 × 101 | 3.8 | 8 | 3.5 |
1:256 | 1 | 3.9 | 2.6 | ND | ND | 2 | 2.9 |