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Table 6 Identification of positive DBS samples by a two-stage hierarchy pooling scheme (pool size = 3)

From: Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies

Pool ID

Result of each pool (log10 copies/mL blood)

Sample ID

Result of each member (log10 copies/mL blood)

Previously reported result

Pf qRT-PCR

Pan qRT-PCR

Pf qRT-PCR

Pan qRT-PCR

1–15, 17, 19, 20, 22, 23, 26

ND

ND

n/a

n/a

n/a

ND

Pool 16

5.35

5.43

Z076

ND

ND

ND

Z077

ND

ND

ND

Z078

5.97

6.16

Pf

Pool 18

3.87

3.57

Z082

ND

ND

ND

Z083

ND

ND

ND

Z084

3.87

4.17

Pf

Pool 21

ND

5.65

Z093

ND

ND

ND

Z094

ND

6.27

Non-Pf Plasmodium sp.

Z095

ND

ND

ND

Pool 24

ND

5.43

Z102

ND

6.03

Non-Pf Plasmodium sp.

Z103

ND

ND

ND

Z104

ND

ND

ND

Pool 25

5.02

5.23

Z105

ND

ND

ND

Z106

ND

ND

ND

Z107

5.41

5.86

Pf

  1. Pf P. falciparum, Pan pan-Plasmodium
  2. ND not detected, n/a not applicable