Adaptation of ELISA detection of Plasmodium falciparum and Plasmodium vivax circumsporozoite proteins in mosquitoes to a multiplex bead-based immunoassay

Table 2 Lower limits of sporozoite detection for circumsporozoite (cs) enzyme-linked immunosorbent assay (ELISA) and cs multiplex-bead assay (MBA) extrapolated from best fit curve of twofold dilution series and rounded to nearest 25 for P. falciparum, P. vivax210 and P. vivax247

	Lower limit of detection (# sporozoites/mosquito equivalent)			Fold-increase in csMBA sensitivity
	csELISA Previously reported	csELISA Current findings	csMBA	Fold-increase in csMBA sensitivity
P. falciparum	125 [2]	1400	25	56
P. vivax210	125 [4]	425	25	17
P. vivax247	250 [4]	1650	25	66

Fold-increase represents the csMBA limit of sporozoite detection compared to csELISA current findings. Previously published limits of sporozoite detection reported as sporozoites per 50 μl of mosquito homogenate, adjusted to "per mosquito equivalent" by multiplying by 5 are included

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