Table 3 Circumsporozoite (cs) multiplex-bead assay (MBA) lower limit of sporozoite detection for Plasmodium falciparum, P. vivax210 and P. vivax247 determined using three methods: A—traditional cut-off calculation method of two times the average (Avg) median fluorescence intensity minus background (MFI-Bkgd) of the negative control wells and two alternative methods: B—the average MFI-Bkgd of the negative control wells plus three standard deviations (SD) and C—MFI-Bkgd values greater than 100
Cut-off determination method | csMBA lower limit of detection (# sporozoites/mosquito equivalent) | ||
|---|---|---|---|
P. falciparum | P. vivax210 | P. vivax247 | |
2 × Avg of negatives | 5 | 4 | 13 |
Avg of negatives + 3SD | 9 | 10 | 32 |
MFI > 100 | 12 | 6 | 124 |