Fig. 3
SJ733 targets mature life-stages for early removal, with a partial requirement for phagocytes. A Representative FACS plots showing life-stage gating for rings, trophozoites and schizonts in Gen0 pRBC over time. Mice (n = 6/group) were administered CTFR+ RBC harbouring P. berghei-GFP (termed Gen0), treated immediately with control saline, sodium artesunate or SJ733, and peripheral blood monitored individually at times indicated by flow cytometry. B Summary dynamics for Gen0 rings, trophozoites and schizonts expressed as a percentage of all RBC. C Schematic the experimental approach. Mice (n = 5/group) were phagocyte-depleted treated with clodronate liposomes 3 days prior to the transfer of CTFR+ RBC harbouring P. berghei-GFP. Mice were then immediately treated with SJ733 or saline control, and flow cytometric assessment of Gen0 and Gen1+ pRBC was performed at the times indicated. D Summary of drop in Gen0 pRBC levels over the first 7 h. Error bars represent the standard the standard error of the mean