Blood-feeding patterns of Anopheles vectors of human malaria in Malawi: implications for malaria transmission and effectiveness of LLIN interventions

Table 1 Primers and probes for three species within the An. funestus species assemblage and their blood-meal hosts

Organism	Nucleotide sequence (5′-3′)
An. funestus	Forward: AGA ACA CTA TGG CGA GCA GC
	Reverse: TTA CGA CGG ATA CGG TCA ACG
	Funestus probe: FAM-CAT GGG GAA ATT CAA TCG AAA ACC TCT-QSY
	Parensis probe: ABY-TGG CGT GCT CGG AAC CTA GC-QSY
	Vaneedeni probe: VIC-CGT TGT GAA AAA TGG AGA TTC ATT TGA AAA CC-QSY
Human	Forward: GGC CTG TTC CTC CCT TAT TT
	Reverse: TAC ACA GGG CTT CCG AGT
	Probe: FAM-ATG GAG TCT GTG TTC CCT GTG ACC-QSY
Goat	Forward: TAG GCG CCA TGC TAC TAA TTC
	Reverse: GAG TGG ATT TGC TGG GAT ATA G
	Probe: VIC-ATT CAC ACC CGA CCT ACT CGG AGA-QSY
Dog	Forward: TGG ACA AAG CAA CCC TAA CA
	Reverse: CCG GTT TCG TGT AGA AAT AGG A
	Probe: ABY-TCA TCC TCC CTT TCA TCA TCG CAG C-QSY
Vertebrates	Forward: CCC CTC AGA ATG ATA TTT GTC CTC A
Vertebrates	Reverse: CCA TCC AAC ATC TCA GCA TGA TGA AA

ISSN: 1475-2875