Table 1 Primer information
From: The ecdysone receptor regulates several key physiological factors in Anopheles funestus
Name | Sequence (5’-3’) | Tm (°C) | Amplicon size (base pairs) | Reference |
|---|---|---|---|---|
An. funestus EcR (forward) | GAT TCT TCC GAC GTA GTG TG | 60 | 484 | Designed in this study |
An. funestus EcR (reverse) | TCC TCG TTG GGT GAG TTA | 60 | ||
An. funestus T7-EcR (forward) | TAA TAC GAC TCA CTA TAG GGA GAG ATT CTT CCG ACG TAG TGT G | 67 | 530 | Designed in this study |
An. funestus T7-EcR (reverse) | TAA TAC GAC TCA CTA TAG GGA GAT CCT CGT TGG GTG AGT TA | 68 | ||
T7-GFP (forward) | TAA TAC GAC TCA CTA TAG GGA GAA CGT AAA CGG CCA CAA GT | 66.5 | 544 | Designed by Ms. E. Ekoka (WRIM) |
T7-GFP (reverse) | TAA TAC GAC TCA CTA TAG GGA GAG GGT GTT CTG GTA GTG | 68.4 | ||
qPCR EcR (forward) | GCC GGT AGC ACA AGT AAT AG | 60 | 130 | Designed in this study |
qPCR EcR (reverse) | GAT CGA GCA TTC CGA CAG | 60 | ||
LRIM9 (forward) | CAG TTC TTC ACC GCA TAG TT | 60 | 117 | Designed in this study |
LRIM9 (reverse) | TTG TCG TCC AGG TAG AGT T | 60 | ||
Lp (forward) | GCT TCG ACA AGG TGT TAG AG | 60 | 104 | Designed in this study |
Lp (reverse) | AAG ACC AAG AGC GGT AGT | 60 | ||
VgR (forward) | TAC TTA CGG CGG GAC TTA T | 60 | 147 | Designed in this study |
VgR (reverse) | GGA GCT GAT CCT GTA TGA TTG | 60 | ||
RPS7 (forward) | TTA CTG CTG TGT ACG ATG CC | 60.4 | 134 | Amenya et al. [14] |
RPS7 (reverse) | GAT GGT GGT CTG CTG GTT | 62.3 | ||
RPL19 (forward) | GAA ACA CCA ACT CCC GAC A | 60.2 | 223 | Spillings et al. [6] |
RPL19 (reverse) | TCA ACA GGC GAC GCA ACA CA | 62.3 |