Fig. 4

Comparison of XN-31 scattergrams of a true positive Plasmodium falciparum sample with a false-positive MI-RBC sample. A Scattergram of a true positive MI-RBC samples containing erythrocytes infected with Plasmodium falciparum. B Scattergram of a sample of a patient in sickle crisis that produced a false positive MI-RBC result by the XN-31. The red particles are what the XN-31 suspects to be a Plasmodium infected red blood cell, the teal particles are the leukocytes, and the dark blue particles are the non-infected red blood cells or debris. In A, a true positive sample, the cluster of events is vertical (green circles), whereas the false-positive events identified in the MI-RBC channel in the sickle cell crises samples cluster at a 45° angle (orange circle in panel B). FSC: forward scatter light; SFL: side-fluorescent