A reverse vaccinology approach on transmembrane carbonic anhydrases from Plasmodium species as vaccine candidates for malaria prevention

Table 2 Prediction of affinity of different HLAs of MHC-II alleles against top domains of α- and η-CA protein sequences from Plasmodium spp.

CA class	MHC-II type	Peptide sequence	Affinity (0 ≤ X ≤ 1)	Antigenicity prediction	Result^d
α-CA^a (group 1) (W7JAI7)	DRB1	428-LRTIINVSSAVHVGS-442	0.811	428-LRTIINVSSAVHVGSDPTLVELK-450	SB
α-CA^a (group 1) (W7JAI7)	DRB1	468-VFPIQVLISSAISNI-482	0.873	461-RKFSLVQVFPIQVLISSAIS-480	SB
α-CA^b (group 2) (Q8IHW5)	DRB1	424-SEKFLRTIINVSSAV-438	0.732	428-LRTIINVSSAVHVGSDPTLVELK-450	SB
η-CA^c (V7PFH4)	DQ	629-YGRVYLIQGFPVQLL-643	0.544	629-YGRVYLIQGFPVQLLISSALT-649	SB

^aMHC II HLA-DRB3, -DRB4, -DRB5, -DP, and -DQ did not show any affinity to group 1 (W7JAI7) of α-CA from P. falciparum
^bMHC II HLA-DRB3, -DRB4, -DRB5, -DP, and -DQ did not show any affinity to group 2 (Q8IHW5) of α-CA from P. falciparum
^cMHC II HLA-DRB1, -DRB3, -DRB4, -DRB5, and -DP did not show any affinity to η-CA (V7PFH4) from P. yoelii
^dSB strong binding peptides (the default value is IC₅₀ ≤ 50 nM or %-Rank ≤ 0.5). IC₅₀ values in nM and %-Rank are the results after the prediction using NetMHCpan 2.8 [100]

ISSN: 1475-2875