A microfluidic paper analytical device using capture aptamers for the detection of PfLDH in blood matrices

Malaria Journal

Table 1 Summary of aptamers reported previously to bind to PLDHs that were used in this study

Aptamer	Reported sequence, sourced for this study (5′-3′)	Binding buffer used	Reference
LDHp1	GCCTGTTGTGAGCCTCCTAACCAGGAAGCGACCTACTAAAGTGATATTAT AGATTCACGGGAGCGTGGTGCATGCTTATTCTTGTCTCCC	2 mM HEPES, 0.2 mM MgCl₂, 0.2 mM CaCl₂, 0.2 mM KCl, 15 mM NaCl, pH 7.4	[12]
LDHp11	GCCTGTTGTGAGCCTCCTAACCTACTGTTGATATGAGTGATAGGGCGGCG CGCTTATCTAGTGTATTGTGCATGCTTATTCTTGTCTCCC
rLDH4	GCCTGTTGTGAGCCTCCTAACCAGCTCGTAGAAAAAAAAAGATATTGCTTCAATTATCTCCTCGCGTTCAATTAACCCAGCATGCTTATTCTTGTCTCCC
rLDH7	GCCTGTTGTGAGCCTCCTAACCCAGAATAGGGACTGCTCGGGATTGCGGA TGAGTCTGGGTGGGACATGGCATGCTTATTCTTGTCTCCC
rLDH15	GCCTGTTGTGAGCCTCCTAACTTTAAAGTTGCTATTTAACCAAAAAAAAAAAAATAAAAAAGTCGAGCCGGCCCATGCTTATTCTTGTCTCCC
pL1	CACCTAATACGACTCACTATAGCGGATCCGACTCACGTACAGCAAGGTTC GATTGGATTGTGCCGGAAGTGCTGGCTCGAACAAGCTTGC	20 mM Tris–HCl, 50 mM NaCl, 5 mM KCl, 5 mM MgCl₂, 300 mM imidazole, pH 8.0	[10]
2008s	CGTACGGTCGACGCTAGCCTGGGCGGTAGAACCATAGTGACCCAGCCGTC TACCACGTGGAGCTCGGATCC	10 mM Na₂HPO₄, 1.8 mM KH₂HPO₄, 2.7 mM KCl, 137 mM NaCl, pH 7.4	[11]

Bolded sections of the reported sequences refer to primer-binding sites used during SELEX, while unbolded sequences refer to the variable regions.

ISSN: 1475-2875