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Fig. 3 | Malaria Journal

Fig. 3

From: Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11

Fig. 3

Copper binding to rMBPPfCox11Ct and mutants in vitro measured by the BCA release assay. Affinity purified rMBPPfCox11Ct or mutants C60A, C157A, C60A–C157A or rMPB were incubated with CuCl2 in the absence (−) or presence ( +) of ascorbic acid in vitro. Copper was detected with the BCA release assay without (open bars) or with (closed bars) the addition of ascorbic acid. Copper chloride was equimolar to protein. Results are the means ± SE of triplicate measurements from duplicate samples. A two-way ANOVA with Bonferroni multiple comparison tests were conducted on the results of samples incubated with CuCl2 in the presence of ascorbate. Comparisons are between rMBPPfCox11Ct and mutants and rMPB where *P < 0.001

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Malaria Journal

ISSN: 1475-2875

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