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Table 1 Purification table for rMBPPfCox11Ct

From: Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11

Sample Total vol (ml) Total proteina (mg) Yield (%) Yield (mg/g pellet)
Soluble E. coli lysateb 30.15 ± 1.91c 122.41 ± 2.20 100 42.07 ± 0.76
Unbound affinity column fraction 27.46 ± 2.06 103.33 ± 0.70 84.41 ± 2.09 35.51 ± 0.24
Washes 58.50 ± 7.78 11.08 ± 1.10 9.04 ± 0.74 3.81 ± 0.38
Affinity purified rMBPPfCox11Ct 23.59 ± 1.47 1.43 ± 0.19 1.17 ± 0.18 0.49 ± 0.06
  1. aProtein determined by the Bradford protein assay (see Expression and purification of recombinant MBPPfCox11Ct and mutants)
  2. bOriginal cell pellet = 2.91 ± 0.22 g
  3. cData presented are Mean ± SD values from duplicate purifications