Expression and copper binding characteristics of Plasmodium falciparum cytochrome c oxidase assembly factor 11, Cox11

Table 1 Purification table for rMBPPfCox11Ct

Sample	Total vol (ml)	Total protein^a (mg)	Yield (%)	Yield (mg/g pellet)
Soluble E. coli lysate^b	30.15 ± 1.91^c	122.41 ± 2.20	100	42.07 ± 0.76
Unbound affinity column fraction	27.46 ± 2.06	103.33 ± 0.70	84.41 ± 2.09	35.51 ± 0.24
Washes	58.50 ± 7.78	11.08 ± 1.10	9.04 ± 0.74	3.81 ± 0.38
Affinity purified rMBPPfCox11Ct	23.59 ± 1.47	1.43 ± 0.19	1.17 ± 0.18	0.49 ± 0.06

^aProtein determined by the Bradford protein assay (see Expression and purification of recombinant MBPPfCox11Ct and mutants)
^bOriginal cell pellet = 2.91 ± 0.22 g
^cData presented are Mean ± SD values from duplicate purifications

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