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Fig. 1 | Malaria Journal

Fig. 1

From: Inhibitory effects of anthracyclines on partially purified 5′–3′ DNA helicase of Plasmodium falciparum

Fig. 1

Partial purification of native 5′ to 3′ DNA helicase from P. falciparum crude extract. A Parasite crude extract was purified on the Resource Q column with 0–100% KCl linear gradient. B Resource Q pooled fraction containing helicase activity was purified on the Mono S column with 0–100% KCl linear gradient. C Mono S pooled active fraction was further purified on the ssDNA column with 0–100% KCl linear gradient. Each fraction was measured for the amount of proteins by using the Bradford reagent and assayed for unwinding activity using the M13-17-mer substrate

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Malaria Journal

ISSN: 1475-2875

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