Streamlining sporozoite isolation from mosquitoes by leveraging the dynamics of migration to the salivary glands

Malaria Journal

Table 1 The full and minimal model of sporozoite yields after stepwise elimination

Dependent variable (y) →		Sporozoites / salivary gland (‘mean sporozoite yields’)
Dependent variable (y) →		Full model			Minimal model
Row	Predictors	Z-value	p	Log-mean (se)	Z-value	p	Log-mean (se)
1	(Intercept)	54.17	< 0.001	7.85 (0.14)	66.63	< 0.001	7.91 (0.12)
2	Mean oocyst densities [linear]	5.52	< 0.001	6.15 (1.11)	6.17	< 0.001	5.97 (0.97)
3	Mean oocyst densities [quadratic]	− 2.22	0.027	− 2.57 (1.16)	− 2.83	0.005	− 2.81 (0.99)
4	Days post-bloodmeal [linear]	2.62	0.009	2.14 (0.82)	2.73	0.006	1.5 (0.55)
5	Days post-bloodmeal [quadratic]	0.49	0.623	0.28 (0.56)
6	PbGFP-LUC_CON [vs PbANKA]	0.38	0.707	0.14 (0.37)
7	Mean oocyst densities * Days post-bloodmeal [linear]	− 1.01	0.312	− 5.79 (5.73)
8	PbGFP-LUC_CON * Mean oocyst densities [vs. PbANKA * Mean oocyst densities]	− 0.08	0.938	− 0.31 (4.00)
9	PbGFP-LUC_CON * days post-bloodmeal [vs. PbANKA * days post-bloodmeal]	− 1.10	0.270	− 1.81 (1.64)
10	PbGFP-LUC_CON * Mean oocyst densities * days post-bloodmeal [vs. PbANKA * Mean oocyst densities * days post-bloodmeal]	1.24	0.215	21.01 (16.95)
Random effects
11	σ_ϵ²	0.27			0.28
12	τ₀₀ (groups)	0.37			0.34
13	ICC (%)	0.57			0.55
14	N (groups)	46			46
15	Observations	75			75
16	Marginal R²/Conditional R² (%)	0.476/0.777			0.475/0.763

Sporozoite yields are dependent on (arithmetic) mean oocyst densities and time. Yields do not represent a true average as sporozoites were recovered by pooling salivary glands without any information of densities in individuals, or infection status (sporozoites ≥ 0). For maintaining consistency with the latter, mean oocyst densities were estimated from midguts irrespective of infection status (i.e., oocysts ≥ 0)

ISSN: 1475-2875