Table 3 Primers for gel-based PCR [17, 49, 50]

Plasmodium sp. Genus Specific

PLU1

TCA AAG ATT AAG CCA TGC AAG TGA

PLU3

TTT TTA TAA GGA TAA CTA CGG AAA AGC TGT

PLU4

TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC

PLU5

CTT GTT GTT GCC TTA AAC TTC

PLU6

TTA AAA TTG TTG CAG TTA AAACG

PLU Cal 2

ACA CAW RGT KCC TCT AAG AAG C

P. falciparum (~ 206 bp)

rFAL1

TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT

rFAL2

ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC

P. vivax (~ 205 bp)

rVIV6

TAA CGC CGT TAG CTA GAT CCA CAA GG

rVIV7

CTG TAG TAT TCA AAA CGC GCA ATG CTG

P. malariae (~ 145 bp)

rMAL1

ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC

rMAL2

AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA

P. ovale (~ 419 bp)

rOVA1

ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA

rOVA4

ACT GAA GGA AGC AAT CTA AGA AAT TT

P. knowlesi (~ 279 bp)

Kn1f

CTC AAC ACG GGA AAA CTC ACT AGT TTA

Kn3r

GTA TTA TTA GGT ACA AGG TAG CAG TAT GC

P. inui (~ 479 bp)

PinF5

GTA TCG ACT TTG TGC GCA TTT TTC TAC

INAR3

GCA ATC TAA GAG TTT TAA CTC CTC

P. cynomolgi (~ 137 bp)

CY2F

GAT TTG CTA AAT TGC GGT CG

CY4F

CGG TAT GAT AAG CCA GGG AAG T

P. coatneyi (~ 505 bp)

PctF1

CGC TTT TAG CTT AAA TCC ACA TAA CAG AC

PctR1

GAG TCC TAA CCC CGA AGG GAA AGG