Deletion patterns, genetic variability and protein structure of pfhrp2 and pfhrp3: implications for malaria rapid diagnostic test in Amhara region, Ethiopia

Table 3 Genetic diversity of pfhrp2 and pfhrp3 by geographical location

Health centre	No. of isolates	S	H	K	π	Θ per site	Θ per sequence
Pfhrp2 gene
Overall	95	532	92	63.36	0.158	0.162	135.53
Tis Abay	57	419	55	61.32	0.150	0.147	108.07
Bahir Dar	16	305	16	65.13	0.175	0.180	108.73
Zenzelima	3	532	3	63.36	0.159	0.162	135.54
Meshenti	19	320	19	65.46	0.163	0.151	114.65
Pfhrp3 gene
Overall	79	188	77	10.223	0.027	0.083	49.02
Tis Abay	50	140	48	9.01	0.025	0.071	41.75
Bahir Dar	13	91	13	13.41	0.035	0.068	34.29
Meshenti	16	57	16	10.11	0.023	0.049	25.06

Results were obtained using pairwise comparison, 4,465 pairwise comparisons have been performed and the average number of sites analysed by comparison was 405,72, total number of sites were 916
S: number of segregating sites; H: number of different haplotypes; K: average number of nucleotide differences; π: nucleotide diversity; Θ per site: genetic diversity per site; Θ per sequence: genetic diversity per sequence

ISSN: 1475-2875