Table 1 Estimates of malaria parasitemia prevalence among rapid diagnostic test negative samples by individual and one-step pooled testing by quantitative polymerase chain reaction

Asembo

Individual

Binomial

1735

189

1

ref.

ref.

10.9 (9.5–12.5)

One-step pooled

MLE, se = sp = 1

347

116

5

79.2 (72.5–85.8)

99.0 (97.7–100.0)

7.8 (6.4–9.2)

One-step pooled

MLE, se = 0.792, sp = 0.990

347

116

5

10.2 (8.2–12.1)^a

Gem

Individual

Binomial

2145

303

1

ref.

ref.

14.1 (12.7–15.7)

One-step pooled

MLE, se = sp = 1

429

164

5

73.7 (67.9–79.6)

98.1 (96.3–99.9)

9.2 (7.8–10.5)

One-step pooled

MLE, se = 0.737, sp = 0.981

429

164

5

13.2 (10.9–15.5)^a

Karemo

Individual

Binomial

790

145

1

ref.

ref.

18.4 (15.8–21.2)

One-step pooled

MLE, se = sp = 1

158

73

5

69.6 (60.7–78.5)

96.4 (91.6–100.0)

11.7 (9.1–14.2)

One-step pooled

MLE, se = 0.696, sp = 0.964

158

73

5

18.7 (13.3–24.1)^a

Overall

Individual

Binomial

4670

637

1

ref.

ref.

14.5 (13.6–15.3) ^b

One-step pooled

MLE, se = sp = 1

934

353

5

74.5 (70.5–78.5)

98.3 (97.1–99.5)

9.5 (8.5–10.5) ^b

One-step pooled

MLE, se = 0.745, sp = 0.983

934

353

5

13.9 (12.6–15.2) ^a,b