Study area | qPCR testing strategy | Prevalence estimation method | Samples or pools tested, N | Positive, n | Pool size | Sensitivity, % (95% CI) | Specificity, % (95% CI) | Prevalence, % (95% CI) |
---|---|---|---|---|---|---|---|---|
Asembo | Individual | Binomial | 1735 | 189 | 1 | ref. | ref. | 10.9 (9.5–12.5) |
 | One-step pooled | MLE, se = sp = 1 | 347 | 116 | 5 | 79.2 (72.5–85.8) | 99.0 (97.7–100.0) | 7.8 (6.4–9.2) |
 | One-step pooled | MLE, se = 0.792, sp = 0.990 | 347 | 116 | 5 |  |  | 10.2 (8.2–12.1)a |
Gem | Individual | Binomial | 2145 | 303 | 1 | ref. | ref. | 14.1 (12.7–15.7) |
 | One-step pooled | MLE, se = sp = 1 | 429 | 164 | 5 | 73.7 (67.9–79.6) | 98.1 (96.3–99.9) | 9.2 (7.8–10.5) |
 | One-step pooled | MLE, se = 0.737, sp = 0.981 | 429 | 164 | 5 |  |  | 13.2 (10.9–15.5)a |
Karemo | Individual | Binomial | 790 | 145 | 1 | ref. | ref. | 18.4 (15.8–21.2) |
 | One-step pooled | MLE, se = sp = 1 | 158 | 73 | 5 | 69.6 (60.7–78.5) | 96.4 (91.6–100.0) | 11.7 (9.1–14.2) |
 | One-step pooled | MLE, se = 0.696, sp = 0.964 | 158 | 73 | 5 |  |  | 18.7 (13.3–24.1)a |
Overall | Individual | Binomial | 4670 | 637 | 1 | ref. | ref. | 14.5 (13.6–15.3) b |
 | One-step pooled | MLE, se = sp = 1 | 934 | 353 | 5 | 74.5 (70.5–78.5) | 98.3 (97.1–99.5) | 9.5 (8.5–10.5) b |
 | One-step pooled | MLE, se = 0.745, sp = 0.983 | 934 | 353 | 5 |  |  | 13.9 (12.6–15.2) a,b |