Table 4 Details of P. falciparumK-13 propeller gene (Pfk13), P. falciparum chloroquine-resistance transporter (Pfcrt) and P. falciparum multi drug resistance-1(Pfmdr-1) nucleotide fragments and population genetic parameters in P. falciparum field isolates of Odisha, India
Gene (Size of the Reference Sequence) | Pfcrt (3107Â bp) | Pfmdr-1 (4382Â bp) | PfK13 ( 2417Â bp) |
|---|---|---|---|
Nucleotide positions | 403,222–406,317 | 957,890–962,149 | 1,724,817–1,726,997 |
Total number of isolates | 17 | 8 | 9 |
Size of the fragment (nucleotide) | 212 | 526 | 793 |
Total number of polymorphic (segregating) sites | 4 | 2 | 6 |
Total number of singleton mutations | 1 | 1 | 2 |
Total number of SNPs | 4 | 2 | 6 |
Total number of haplotypes | 5 | 3 | 5 |
Haplotype diversity | 0.64 | 0.679 | 0.722 |
Nucleotide diversity π | 0.00472 | 0.00156 | 0.00266 |
Nucleotide diversity (Ï´) | 0.00558 | 0.00147 | 0.00278 |
Tajima’s D | − 0.47378 | 0.24178 | − 0.19073 |
Fu and Li’s D | 0.23149 | − 0.14931 | 0.26719 |
Fu and Li’s F | 0.04712 | − 0.06487 | 0.17557 |