Fig. 3

Plasmodium falciparum and P. vivax FT-GPI-detection of reference GPI-AP. Each protein listed in the reference set (Table 1) was tested using 31 different combinations of FT-GPI parameter values (Additional file 2: Table S2). For each protein, the horizontal-colored bar depicts the distinct number of parameters sets yielding prediction of GPI-AP + (as given in Additional file 6: Table S6). Of particular note for follow-up study are the six proteins at the bottom of the list, which are new putative GPI-APs detected by FT-GPI and discussed herein. MSP1p from P. vivax and MSP2 from P. falciparum had no ortholog in the corresponding species. Plasmodium falciparum P36 ortholog (not shown) was not in the list reported by Gilson, et al. [17] and was classified as GPI-AP- by all FT-GPI parameter sets. Some proteins had no gene names and were arbitrarily labelled from gpi1 to gpi4. The P. vivax ortholog of Pf_gpi1 was absent from the list reported by Carlton, et al. [38] and was also GPI-AP- using FT-GPI analysis. The gpi3 protein was labelled as P32 in some references. FT-GPI classified all SRAs as GPI-AP- with all parameter sets. MOLO1_domain-containing protein encoding genes in P. falciparum and P. vivax reference sets were in fact paralogs. CA: carbonic anhydrase; UNK mean that the protein was of unknown function t