Fig. 4

P. falciparum and P. vivax proteins classified as GPI-AP + by FT-GPI when using both PLA001 or PLA030 parameter sets (which tended to be the most restrictive sets). A set of 27 GPI-APs was confirmed in P. falciparum 3D7. There were 28 confirmed GPI-APs in P. vivax Sal-1 analysis. Twenty-one proteins were conserved between the initial prediction of P. falciparum and P. vivax [17, 38] and FT-GPI detection using PLA001 or PLA030. Plasmodium falciparum label is for P. falciparum proteins whose ortholog in P. vivax is not GPI-AP. Plasmodium vivax indicated P. vivax proteins with ortholog in P. falciparum that was not GPI-AP. Proteins with no ortholog in P. falciparum or P. vivax are marked with *. Proteins expressed at a conserved locus, but with different OrthoMCL ID, are in bold. The P. falciparum 3D7 GPI-proteome is composed of 24 previously described GPI-APs [17] and three new putative GPI-APs. The P. vivax Sal-1 GPI-proteome composition encompassed 24 previously-identified GPI-APs [38] and four new proteins. The 6-cystein protein and corresponding OrthoMCL IDs are in green. Members of the MSP multigene family are in blue. Ookinete proteins are in violet