Fig. 6

Using FT-GPI configured with PLA030 to guide investigation of the evolution of the GPI-Proteome among Haemosporida. Gene-encoding proteins with a size over 210 aa were selected for this analysis. A. Heatmap representing the distribution of genes among species. The presence of orthologs and paralogs was established using OrthoMCL annotation. Only orthology groups presenting orthologs in more than four species were included in the present analysis. The presence of paralogs were detected for some genes and are quantified in red. A GPI-AP was absent (white) either because it was not detected by FT-GPI using PLA030, or the gene was absent from the genome. Some genes were represented by more than one OrthoMCL group. The discrepancy between synteny and orthology groups may be due to rapid sequence evolution and shared homologies. The complete species name is given in panel B and Additional file 3: Table S3. Laverania-Pg differentiated P. gaboni and close species from the P. falciparum/P. reichenowi group of parasites [68]. B. Evolution of the GPI-proteome is related to speciation. With the same sets of genes as in Panel A, the number of paralogs was set to 1 to compute Jaccard distance. The Ward-2 method was used to build the tree