Fig. 2
Transcriptomic profile of KAE609-treated and PA21A092-treated P. falciparum. A Dimensionally reduced transcriptomic data from untreated wildtypes (WT), PA21A092-treated wildtypes (WT + 12 nM PA92), PA21A092-treated mutants (MT + 400 nM PA92), and KAE609-treated mutants (MT + 30 pM KA09). Markers with lightest shade of a colour denotes 0 h time point, while the darkest shade of a colour denotes 48 h time point. Irrespective of the treatment compound, data from each time point group together (ellipses), except for the 48 h data from KAE609-treated mutants (arrow). B Time occurrence of the maximum Pearson correlation coefficient across the intraerythrocytic developmental cycle (IDC) of the hourly sampled data of Llinas et al. [20] (ordinate) with the culture systems studied here (abscissa). If the untreated and the treated parasites in the culture systems studied here progressed at a rate identical to the experiments of Llinas et al. [20], then the black, red, blue, and green markers would appear on top of each other along the grey line. It was found that the sublethal doses of 400 nM PA21A092 and 30 pM KAE609, respectively, caused minor perturbations to the IDC progression of the wildtypes (red circle) and the mutants (blue triangle, green triangle). C Significantly altered gene abundances in PA21A092-treated and KAE609-treated mutant parasites. The green markers denote log2 (FCgene) >  + 2 and red markers represent log2 (FCgene) < − 2. Here, FCgene denotes fold change in average expression of a gene due to the treatment relative to its average expression in the parental Dd2 parasite. The average is based on 1000 bootstrap calculations (see Methods). αβH, alpha–beta hydrolase; h.p.i., hours post infection; KA09, KAE609 compound; PA92, PA21A092 compound; PFN profiling, PM8 plasmepsin VIII