Fig. 4

PCR-artificial Restriction Fragment Length Polymorphisms genotyping of P. falciparum cytochrome b Y268S mutation. Digestion reactions with NsiI (left) and PstI (right) of 6 representatives of all field isolates (1–6), 3D7 and C2B strains are shown. Digestion reaction (D) and no digestion controls (C) were run in parallel. All isolates and the 3D7 strain were wild type (268Y) as indicated by the digested 359 bp band in the NsiI reaction, but a non-digested 381 bp band in the PstI reaction. The C2B strain was confirmed to have a mutant 268S, defined as the digested band in the PstI reaction, but a non-digested band in the NsiI reaction.