Table 1 Primer sequences, annealing temperature and the expected PCR product size
Primer name | 5′-3′ sequences* | Tm | Size (bp) (bp) |
|---|---|---|---|
PfCYTB33 (F) | ATT TAT GAT ATT TAT TGT AAC TGC | 55 °C (1st round PCR) | 565 |
PfCYTB4R (R) | AGT TGG TTA AAC TTC TTT GTT CTG C | ||
CytbY268S-F2 (F) | AGC AGT AAT TTG GAT ATG TGG AGG | 58 °C (2nd round PCR) | 381 |
CytB_268Y_RFLP (R) (R) (R) | TTA CTT GGA ACA GTT TTT AAC AAT GC | ||
CytB_268S_RFLP (R) | TTA CTT GGA ACA GTT TTT AAC ACT GC |