PET-PCR reveals low parasitaemia and submicroscopic malarial infections in Honduran Moskitia

Malaria Journal

Table 1 List of primers used for amplification reactions, nucleotide sequences, annealing temperatures, and amplicon sizes

Reaction	Primer	Sequence (5′–3′)	Annealing temperature (ºC)	Product size (bp)
First PCR for Plasmodium spp.	rPLU1	TCA AAG ATT AAG CCA TGC AAG TGA	55
First PCR for Plasmodium spp.	rPLU5	CCT GTT GTT GCC TTA AAC TYC
Second PCR for Plasmodium spp.	rPLU3	TTT YTA TAA GGA TAA CTA CGG AAA AGC TGT	62	240
Second PCR for Plasmodium spp.	rPLU4	TAC CCG TCA TAG CCA TGT TAG GCC AAT ACC
PCR for P. vivax	rVIV1	CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC	58	117
PCR for P. vivax	rVIV2	ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA
PCR for P. falciparum	rFAL1	TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT	58	205
PCR for P. falciparum	rFAL2	ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC
PET-PCR for Plasmodium spp.	Genus forward	GGC CTA ACA TGG CTA TGA CG	63	91
PET-PCR for Plasmodium spp.	Labeled–Genus reverse	6FAM- agg cgc ata gcg cct ggC TGC CTT CCT TAG ATG TGG TAG CT

ISSN: 1475-2875