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Table 1 Detection of asexual (18S rRNA) and sexual (Pss25 and Pss48/45) transcripts of Plasmodium in non-human primates from the Atlantic Forest

From: Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest

Target

18S rRNA

Pss25

Pss48/45

Previous PCR result

Amplification

Number of samples (percentage)

Amplification

Number of samples (percentage)

Amplification

Number of samples (percentage)

Ps + samples (single n = 11 or mixed n = 13)

 + 

21 (87.5%)

 + 

13 (62%)

 + 

7 (54%)

−

6 (46%)

−

8 (38%)

−

8 (100%)

−

3 (12.5%)

−

3 (100%)

−

3 (100%)

Pbr + single (n = 5)

 + 

2 (40%)

 + 

1 (50%)

−

1 (100%)

−

1 (50%)

−

1 (100%)

−

3 (60%)

−

3 (100%)

−

3 (100%)

Negative samples (n = 6)

 + 

3 (50%)

−

3 (100%)

−

3 (100%)

−

3 (50%)

−

3 (100%)

−

3 (100%)

  1. The previously published diagnosis is expressed as: positive for P. simium (Ps +), including both single and mixed P. simium infection (with P. brasilianum); positive for P. brasilianum (Pbr +); and parasite-negative samples. For more details about previously published diagnosis please see Alvarenga et al. [31] and Nunes et al. [32]. The amplification results are expressed as positive ( +) or negative (−) for each transcript. Pss25—P. simium sexual antigen 25; Pss48/45—P. simium sexual antigen 48/45