Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest

Malaria Journal

Table 3 Cq values and transcript quantification of the 18S rRNA and Plasmodium simium sexual antigen 25 (Pss25) targets in non-human primate samples

Sample ID	18S rRNA RT-qPCR		Pss25 RT-qPCR		Number of estimated gametocytes/µL^#
Sample ID	Cq	Quantity (copies/µL)	Cq	Quantity (copies/µL)	Number of estimated gametocytes/µL^#
J1	24.3	8.76	33.2	0.07	0.02
J3	20.6	141.22	–
J4	29.5	0.17	–
J5	16.6	2,912.17	29.3	1.12	0.27
J7	17.0	2,248.03	29.2	1.21	0.29
J8**	32.1	0.03
J10*	27.9	0.58	–
J11	13.8	25,170.71	28.0	2.79	0.67
J14	15.1	9,614.21	24.9	24.17	5.80
J15	21.7	65.41	–
J17	18.5	707.94	30.5	0.48	0.12
J18**	32.6	0.02
J20	24.1	10.19	33.9	0.05	0.01
J21*	29.6	0.16	–
J22	23.5	15.98	–
J23**	32.4	0.02	–
J25	21.2	91.59	33.7	0.05	0.01
J26	28.2	0.46	–
J27	17.1	2,065.79	29.8	0.76	0.18
J28	28.0	0.52	–
J29	33.5	0.01	–
J34	31.2	0.05	–
J43	22.7	29.72	–
J44	20.0	225.41	33.8	0.05	0.01
J48	25.5	3.59	–
C2302	31.6	0.04	–

All samples were previously detected as positive for P. simium or mixed infection, except for the indicated samples infected by P. brasilianum (*) or not infected (**). Only samples with Cq < 34 were included for quantification
^#Number of gametocytes estimated according to Koepfli et al. [53]: 1 gametocyte = 4.17 Pss25 transcripts

ISSN: 1475-2875