Composition of Anopheles species and bionomic characteristics over the peak malaria transmission season in Bandarban, Bangladesh

Table 1 Morphological versus molecular identifications

Molecular species	Morphological Identification correct (#)	ITS2			CO1
Molecular species	Morphological Identification correct (#)	NCBI Coverage (%)	NCBI E-value	NCBI % Identity (%)	NCBI coverage (%)	NCBI E-value	NCBI %ID	BOLD % ID
An. baimaii*	100% (n = 54)	97	0	99.59	99	0	98.91	99.84
An. campestris*	90% (n = 10)	98	0	97.75	97	0	97.51	97.5
An. dissidens*	90% (n = 10)	99	0	100	98	0	99.68	100
An. jamesii	63% (n = 16)	98	0	98.89	99	0	99.37	100
An. jeyporensis ^@	90% (n = 21)	99	0	99.72	97	0	99.39	99.84
An. karwari ^@	78% (n = 18)	100	0	99.77	99	0	91.61	99.67
An. kochi ^@	59% (n = 17)	99	0	100	97	0	99.51	99.84
An. maculatus ^@	86% (n = 22)	99	0	100	100	0	99.69	100
An. nivipes ^@	35% (n = 43)	95	0	98.59	99	0	92.13	100
An. peditaeniatus ^@	34% (n = 42)	100	0	100.00	97	0	99.84	100
An. philippinensis ^@	0% (n = 3)	99	0	99.55	–	–	–	–
An. sawadwongporni	0% (n = 6)	99	0	99.30	99	0	93.68	100
An. splendidus ^@	0% (n = 8)	99	0	99.78	–	–	–	–
An. subpictus Form A ^{*, @}	100% (n = 5)	100	0	100	99	0	99.53	99.84
An. subpictus Form B ^{*, @}	100% (n = 1)	100	0	100.00	99	0	99.40	99.21
An. vagus ^{*, @}	92% (n = 13)	99	0	100	99	0	97.90	99.56
An. varuna ^@	53% (n = 23)	100	0	98.92	98	0	98.72	99.18

Seventeen species were identified using ITS2 and CO1 sequences. The percentage with matching morphological identifications and the number of specimens sequenced are noted. Two species did not produce viable CO1 sequences (−). Species identities labelled with * were confirmed using diagnostic PCRs. Species with sequences that matched voucher specimens are marked with ^@

ISSN: 1475-2875