Significant number of Plasmodium vivax mono-infections by PCR misidentified as mixed infections (P. vivax/P. falciparum) by microscopy and rapid diagnostic tests: malaria diagnostic challenges in Ethiopia

Table 3 Over all agreement of RDT and microscopy against real-time PCR as gold standard (n = 365)

Results	Reference Method (PCR)					Agreement	Kappa	Kappa Interpretation/agreement
Results	Pv	Pv/Pf	Pf	Negative	Total	Agreement	Kappa	Kappa Interpretation/agreement
RDT
Pv	310	17	1	1	329	90.41%	0.495	Moderate
Pv/Pf	14	20	1	1	36
Total	324	37	2	2	365
Site level Microscopists
Pv	310	15	1	1	327	90.96%	0.534	Moderate
Pv/Pf	14	22	1	1	38
Total	324	37	2	2	365
Expert Microscopists
Pv	275	19	1	2	297	80.27%	0.238	Fair
Pv/Pf	49	18	1	0	68
Total	324	37	2	2	365

Samples included in this study were those which were positive for P.vivax (Mono or mixed infection) using RDT and microscopy, so P.falciparum (mono) and negative results were not expected from RDT and Microscopy

ISSN: 1475-2875