Therapeutic efficacy of artesunate–amodiaquine and artemether–lumefantrine for the treatment of uncomplicated falciparum malaria in Chad: clinical and genetic surveillance

Issa, Mahamat Souleymane; Warsame, Marian; Mahamat, Moussa Hassane Taisso; Saleh, Issakha Diar Mahamat; Boulotigam, Kodbsse; Djimrassengar, Honoré; Issa, Ali Haggar; Abdelkader, Ousmane; Hassoumi, Manah; Djimadoum, Mbanga; Doderer-Lang, Cécile; Ndihiokubwayo, Jean Bosco; Rasmussen, Charlotte; Menard, Didier

doi:10.1186/s12936-023-04644-w

Table 5 Number and frequency of SNPs detected in genes associated with anti-malarial drug resistance in day0 samples by study site

From: Therapeutic efficacy of artesunate–amodiaquine and artemether–lumefantrine for the treatment of uncomplicated falciparum malaria in Chad: clinical and genetic surveillance

Gene	Codon	AA	No. of sample				% of mutant (%)				p-value
Gene	Codon	AA	Doba	Kelo	Koyom	Total	Doba	Kelo	Koyom	Total	p-value
Kelch13	WT	–	63	78	67	208	1.5	3.7	4.2	3.2	0.4
	N489	489T	0	2	2	4
	A578	578S	1	1	1	3
Pfcrt	72	C	64	81	70	215	0	0	0	0	–
	72	S	0	0	0	0	0	0	0	0	–
	74	M	57	67	61	185	10.9	17.2	12.8	13.9	0.5
	74	I	7	14	9	30	10.9	17.2	12.8	13.9	0.5
	75	N	57	67	61	185	10.9	17.2	12.8	13.9	0.5
	75	E	7	14	9	30	10.9	17.2	12.8	13.9	0.5
	76	K	57	67	61	185	10.9	17.2	12.8	13.9	0.5
	76	T	7	14	9	30	10.9	17.2	12.8	13.9	0.5
	93	T	64	81	70	215	0	0	0	0	–
	93	S	0	0	0	0	0	0	0	0	–
	97	H	64	81	70	215	0	0	0	0	–
	97	Y	0	0	0	0	0	0	0	0	–
	145	F	64	81	70	215	0	0	0	0	–
	145	I	0	0	0	0	0	0	0	0	–
	218	I	64	75	68	207	0	7.4	2.8	3.7	0.2
		M	0	4	0	8
		T	0	1	1
		V	0	1	1
	343	M	64	81	70	215	0	0	0	0	–
	343	L	0	0	0	0	0	0	0	0	–
	350	C	64	81	70	215	0	0	0	0	–
	350	R	0	0	0	0	0	0	0	0	–
	353	G	64	81	70	215	0	0	0	0	–
	353	V	0	0	0	0	0	0	0	0	–
	356	I	60	72	63	195	6.2	11.1	10.0	9.3	0.6
	356	T	4	9	7	20	6.2	11.1	10.0	9.3	0.6
Pfmdr-1	86	N	60	68	61	189	6.2	16.0	12.8	12.0	0.2
	86	Y	4	13	9	26	6.2	16.0	12.8	12.0	0.2
	184	Y	21	29	25	75	67.1	64.1	64.2	65.11	0.9
	184	F	43	52	45	140	67.1	64.1	64.2	65.11	0.9
	970	F	61	76	69	206	4.6	6.1	1.4	4.1	0.5
		I	1	1	1	3
		L	0	2	0	2
		S	0	1	0	1
		V	2	1	0	3
	1034	S	64	81	70	215	0	0	0	0	–
	1034	C	0	0	0	0	0	0	0	0	–
	1042	N	64	81	70	215	0	0	0	0	–
	1042	D	0	0	0	0	0	0	0	0	–
	1246	D	64	81	70	215	0	0	0	0	–
	1246	Y	0	0	0	0	0	0	0	0	–
dhfr	16	A	64	81	7	152	0	0	0	0	–
	16	V	0	0	0	0	0	0	0	0	–
	50	C	64	81	7	152	0	0	0	0	–
	50	R	0	0	0	0	0	0	0	0	–
	51	N	8	13	9	30	87.5	83.9	87.1	86.0	0.8
	51	I	56	68	61	185	87.5	83.9	87.1	86.0	0.8
	59	C	8	6	7	21	87.5	92.5	90.0	90.2	0.6
	59	R	56	75	63	194	87.5	92.5	90.0	90.2	0.6
	108	S	7	3	4	14	89.0	96.2	94.2	93.4	0.2
	108	N	57	78	66	201	89.0	96.2	94.2	93.4	0.2
	164	I	64	81	7	152	0	0	0	0	–
	164	L	0	0	0	0	0	0	0	0	–
dhps	431	I	53	71	60	184	17.1	12.3	14.2	14.4	0.7
	431	V	11	10	10	31	17.1	12.3	14.2	14.4	0.7
	436	S	8	13	20	41	87.5	83.9	71.4	80.9	0.04
	436	A	56	68	50	174	87.5	83.9	71.4	80.9	0.04
	437	G	26	23	36	85	59.3	71.6	48.5	60.4	0.01
	437	A	38	58	34	130	59.3	71.6	48.5	60.4	0.01
	540	K	64	79	70	213	0	2.4	0	0.9	0.2
	540	E	0	2	0	2	0	2.4	0	0.9	0.2
	581	A	64	81	7	152	0	0	0	0	–
	581	G	0	0	0	0	0	0	0	0	–
	613	A	63	81	68	212	1.5	0	2.8	1.4	–
	613	S	1	0	2	3	1.5	0	2.8	1.4	–

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