Table 2 Bioinformatic search results for sequence identity with hCas9
Database | Program | Keyword limitation | Accession # | Organism | Description | Length (aa) | E value | % identity | Alignment length (aa) |
|---|---|---|---|---|---|---|---|---|---|
NCBI-TRS | TBLASTN | Toxin | XM_040336796.1 | Rana temporaria | Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X3, mRNA | 1616 | 0.87 | 38 | 47 |
NCBI-TRS | TBLASTN | Toxin | XM_040336794.1 | Rana temporaria | Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X1, mRNA | 1740 | 0.89 | 38 | 47 |
NCBI-TRS | TBLASTN | Toxin | XM_040336795.1 | Rana temporaria | Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X2, mRNA | 1742 | 2.0 | 36 | 47 |
NCBI-EST | TBLASTN | Toxin | CMD69_G06_40 | Sus scrofa | MNMPM3 Sus scrofa cDNA clone PPSUBLIB_56C06 5', mRNA sequence | 594 | 10.0 | 42 | 38 |
BLAST UniProt-toxin | BLASTP | Toxin | A0A502HM27 | Brevibacillus laterosporus (Bacillus laterosporus) | CRISPR-associated endonuclease Cas9 | 1092 | 2e−25 | 26 | 549 |
BLAST UniProt-toxin | BLASTP | Toxin | H1D476 | Fusobacterium necrophorum subsp. funduliforme | Type II toxin-antitoxin system VapC family toxin | 117 | 2e−18 | 43 | 119 |
BLAST UniProt-toxin | BLASTP | Toxin | A0A1G4RN64 | Lachnospiraceae bacterium | Nucleotidyl transferase AbiEii/AbiGii toxin family protein | 249 | 1.8 | 27 | 103 |
BLAST UniProt-toxin | BLASTP | Toxin | A0A1H2T7K0 | Lachnospiraceae bacterium | Nucleotidyl transferase AbiEii/AbiGii toxin family protein | 249 | 3.9 | 27 | 103 |
BLAST UniProt-toxin | BLASTP | Toxin | A0A2H0VRT0 | Chlamydiae bacterium | Nucleotidyl transferase AbiEii/AbiGii toxin family protein | 253 | 4.5 | 25 | 119 |
BLAST UniProt-toxin | BLASTP | Toxin | A0A7X8NY49 | Citrobacter freundii | Insulinase family protein | 497 | 0.47 | 22 | 168 |
BLAST UniProt-allergen | BLASTP | Allergen | A0A834R755 | Sarcoptes scabiei | Mite allergen Der p 3 | 595 | 3.1 | 30 | 56 |
BLAST UniProt-allergen | BLASTP | Allergen | A0A3S2L044 | Chilo suppressalis | Uncharacterized protein | 194 | 6.9 | 45 | 31 |