Bioinformatic and literature assessment of toxicity and allergenicity of a CRISPR-Cas9 engineered gene drive to control Anopheles gambiae the mosquito vector of human malaria

Qureshi, Alima; Connolly, John B.

doi:10.1186/s12936-023-04665-5

Table 3 Bioinformatic search results for sequence identity with DsRed

From: Bioinformatic and literature assessment of toxicity and allergenicity of a CRISPR-Cas9 engineered gene drive to control Anopheles gambiae the mosquito vector of human malaria

Database	Program	Search restriction	Accession #	Organism	Description	Length (aa)	E value	% identity	Alignment length (aa)	Comments from manual inspection of alignment
BLAST UniProt-Toxin	BLASTP	Toxin	A0A8A1QQ63	Synthetic DNA	DsRed OS = Cloning vector tetO-AaHIT	238	1e−163	98	223	Homologous to fluorescent marker in construct
BLAST UniProt-Toxin	BLASTP	Toxin	A0A8A1QPY2	Synthetic DNA	ZsGreen OS = Cloning vector Op/Ie2-tTAV(OX4585)	247	2e−57	46	205	Homologous to fluorescent marker in construct
BLAST UniProt-Toxin	BLASTP	Toxin	A0A8A1QPH2	Synthetic DNA	ZsGreen OS = Cloning vector Hr5/Ie1-tTAV(OX5378)	247	2e−57	46	205	Homologous to fluorescent marker in construct
BLAST UniProt-Toxin	BLASTP	Toxin	A0A059PIQ0	Aequorea victoria	Green fluorescent protein (Fragment)	251	1e−17	25	218	Homologous to green fluorescent protein
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1I9LJZ6	Stx converting phage vB_EcoS_ST2-8624	Uncharacterized protein	238	1e−17	25	218	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1I9LJ85	Stx converting phage vB_EcoS_P27	Uncharacterized protein	262	2e−17	25	218	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1I9LJQ6	Stx converting phage vB_EcoS_P27	Uncharacterized protein	262	3e−17	25	218	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1I9LJG8	Stx converting phage vB_EcoS_P32	Uncharacterized protein	262	3e−17	25	218	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1I9LJ85	Stx converting phage vB_EcoS_P27	Uncharacterized protein	262	3e−17	25	218	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A0P0UVZ8	Staphylococcus aureus	Toxic shock syndrome toxin	103	1e−04	54	41	Alignment length n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A1M6ERQ6	Flavobacterium terrae	Uncharacterized protein	128	0.80	28	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	F0ICN8	Capnocytophaga sp. oral taxon 338 str. F0234	Uncharacterized protein	128	1.9	28	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A2S0L9N8	Capnocytophaga sp. oral taxon 878	Phage tail protein	128	1.9	28	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	L1P5W9	Capnocytophaga sp. oral taxon 326 str. F0382	Uncharacterized protein	128	2.0	28	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A172XSB0	Chryseobacterium glaciei	Phage tail protein	128	2.2	27	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A502H3U0	Brevibacillus laterosporus	Uncharacterized protein	287	2.4	29	82	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A2S5I5K9	Brevibacillus laterosporus	Toxin-like protein	356	2.4	29	82	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	U2C560	Capnocytophaga sp. oral taxon 863 str. F0517	Uncharacterized protein	128	2.7	28	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A3G6MH72	Chryseobacterium sp. G0201	Phage tail protein	128	2.7	26	115	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A0E9WUE2	Anguilla anguilla	Uncharacterized protein	135	7.2	26	57	Alignment identity n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A345CDE5	Leclercia sp. W17	Type II toxin-antitoxin system RelE/ParE family toxin	98	8.9	35	49	Alignment length n.s
BLAST UniProt-Toxin	BLASTP	Toxin	A0A5Q6S3U2	Mumia sp. Z350	Type II toxin-antitoxin system VapB family antitoxin	98	9.6	28	81	Alignment identity n.s
BLAST UniProt-Allergen	BLASTP	Allergen	A0A1Y2VHK8	Hypoxylon sp. CO27-5	Glycoside hydrolase family 128 protein	461	0.051	25	59	Below 35%/80aa cutoff
BLAST UniProt-Allergen	BLASTP	Allergen	A0A1Y2TLJ6	Hypoxylon sp. EC38	Glycoside hydrolase family 128 protein	461	0.52	28	43	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	ADL32664.1	Daucus carota	Allergen PRP-like protein	154	0.065/1.0	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS Fasta36	Allergen	ADL32662.1	Daucus carota	Allergen PRP-like protein	154	0.15/2.0	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	ADL32665.1	Daucus carota	Allergen PRP-like protein	154	0.18/2.3	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	ADL32666.1	Daucus carota	Allergen PRP-like protein	154	0.18/2.3	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	ADL32663.1	Daucus carota	Allergen PRP-like protein	154	0.22/2.7	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	BAB88129.1	Daucus carota	Allergen pathogenesis-related protein-like	154	0.22/2.7	23.2	125	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	AAS75831.1	Suidasia medanensis	Putative group 2 allergen Sui m 2	141	0.82/7.1	25.9	58	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	BAB21491.1	Betula platyphylla	Allergen Bet vI jap3	160	1.0/8.7	57.9	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAS47037.1	Prunus avium	Allergen Pru av 1	160	1.2	24.4	131	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB06416.1	Daucus carota	Allergen major allergen Dau c 1/1	154	1.4	21.6	125	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	BAA13604.1	Daucus carota	Allergen Dau c 1	154	1.4	21.6	125	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAB01092.1	Daucus carota	Allergen Dau c 1	168	1.7	21.6	125	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07320.1	Betula pendula	Allergen pollen allergen Betv1, isoform at1	120	1.7	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07328.1	Betula pendula	Allergen pollen allergen Betv1, isoform at8	120	1.7	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB03715.1	Daucus carota	Allergen Dau c 1	154	1.8	20.8	125	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAQ24541.1	Blomia tropicalis	Putative Blo t 1 allergen	333	2.4	25.4	67	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	MANUAL2	Blomia tropicalis	Putative Blo t 1.02 Manual Entry	333	2.4	25.4	67	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB03716.1	Daucus carota	Allergen Dau c 1	154	2.7	23.8	126	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	1B6F_A	Betula pendula	Allergen Chain A, Birch Pollen Allergen Bet V 1	159	2.8	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA04829.1	Betula pendula	Allergen pollen allergen, Betv1	159	2.8	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	4BK6_B	Betula pendula	Allergen Chain B, Crystal Structure Of A Dimeri	159	2.8	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	4BK7_A	Betula pendula	Allergen Chain A, Crystal Structure Of A Varian	159	2.8	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07324.1	Betula pendula	Allergen pollen allergen Betv1, isoform at4	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07323.1	Betula pendula	Allergen pollen allergen Betv1, isoform at3	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAD26561.1	Betula pendula	Allergen isoallergen Bet v 1 b2	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB02160.1	Betula pendula	Allergen pollen allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07329.1	Betula pendula	Allergen pollen allergen Betv1, isoform at5	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB02155.1	Betula pendula	Allergen pollen allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA54487.1	Betula pendula	Allergen Bet v 1 j	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB02156.1	Betula pendula	Allergen pollen allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB02157.1	Betula pendula	Allergen pollen allergen Bet v 1 [Betula pe	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA33887.1	Betula pendula	Allergen Bet v 1—like	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAD26560.1	Betula pendula	Allergen isoallergen bet v 1 b1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAS47035.1	Prunus avium	Allergen Pru av 1	160	2.9	25.2	135	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA96547.1	Betula pendula	Allergen major allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA05187.1	Betula pendula	Allergen pollen allergen Betv1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07318.1	Betula pendula	Allergen pollen allergen Betv1, isoform at8	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA96541.1	Betula pendula	Allergen major allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA54484.1	Betula pendula	Allergen Bet v 1 f	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA05188.1	Betula pendula	Allergen pollen allergen Betv1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAB02161.1	Betula pendula	Allergen pollen allergen Bet v 1	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07326.1	Betula pendula	Allergen pollen allergen Betv1, isoform at5	160	2.9	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	BAB21490.1	Betula platyphylla	Allergen Bet vI jap2	160	3.5	55.0	20	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA96545.1	Betula pendula	Allergen major allergen Bet v 1	160	3.5	52.6	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	ACD65080.1	Forcipomyia taiwana	Allergen For t 1	118	3.9	30.2	43	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CBG76811.1	Secale cereale	Putative pollen allergen Sec c 5	202	4.3	24.4	172	Below 35%/80aa cutoff
COMPARE/AllergenOnline	COMPASS/Fasta36	Allergen	P83834.1	Cucumis melo	Allergen Pathogenesis-related protein (PR-1)	41	6.1/2.3	25.6	39	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA04823.1	Betula pendula	Allergen pollen allergen, Betv1	159	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	BAB21489.1	Betula platyphylla	Allergen Bet vI jap1	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AAS47036.1	Prunus avium	Allergen Pru av 1	160	6.5	23.7	131	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA54482.1	Betula pendula	Allergen Bet v 1 d	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07325.1	Betula pendula	Allergen pollen allergen Betv1, isoform at4	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	P43180.2	Betula pendula	Allergen Major pollen allergen Bet v 1-G	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	AJE61290.1	Prunus persica	Allergen Pru p 1	160	6.5	24.4	131	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA54489.1	Betula pendula	Allergen Bet v 1 l	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA05190.1	Betula pendula	Allergen pollen allergen Betv1	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA07319.1	Betula pendula	Allergen pollen allergen Betv1, isoform at1	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA05186.1	Betula pendula	Allergen pollen allergen Betv1	160	6.5	47.4	19	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	COMPARE00288	Gallus gallus	Allergen Gal d 9	45	8.3	70.0	10	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	COMPARE200	Phoenix sylvestris	alpha 1, 4 glucan synthase, partial sequence	12	8.9	71.4	7	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	COMPARE00276	Delonix regia	unknown function, partial	18	9.6	100.0	6	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	1QMR_A	Betula pendula	Allergen Chain A, Birch Pollen Allergen Bet V 1	159	9.8	37.0	46	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAA96542.1	Betula pendula	Allergen major allergen Bet v 1	160	9.9	27.6	116	Below 35%/80aa cutoff
COMPARE	COMPASS	Allergen	CAD10374.1	Castanea sativa	Putative ypr10	160	9.9	21.9	105	Below 35%/80aa cutoff

n.s.: not significant; 35%/80aa cutoff: alignments to allergens are considered significant when at least 35% identical over a segment of 80 or more amino acids

Back to article page

ISSN: 1475-2875

Contact us

Submission enquiries: journalsubmissions@springernature.com

Malaria Journal

Contact us