Fig. 5

Comparison of freezing and storage conditions. (A) Schematic of parasite freezing and storage conditions tested (V1–V4). Comparison of samples Post thaw (B), Post-KCl-Percoll (C), 24-h (D) and 48-h (E) with parasitaemia values normalized to V1. **P < 0.01 via mixed effects analysis with Dunnett’s multiple comparisons. Comparison of samples stored long term (7–10 years) in either liquid nitrogen or at − 80 °C: (F) post-thaw and (G) at 24-h in short-term culture