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Table 1 List of primer mix used for secondary PCR and deep sequencing of msp1 gene (NC_004330.2)

From: Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya

 

Primer sequence

Position

Forward primers mix

5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTAGAAGCTTTAGAAGATGCAGTATTG-3'

1201627

5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNCTAGAAGCTTTAGAAGATGCAGTATTG-3'

5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNCTAGAAGCTTTAGAAGATGCAGTATTG-3'

Reverse primers mix

5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGATTGGTTAAATCAAAGAGTTCGG-3'

1202210

5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNTGATTGGTTAAATCAAAGAGTTCGG-3'

5'-5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNTGATTGGTTAAATCAAAGAGTTCGG-3'

5'- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGNNNTGATTGGTTAAATCAAAGAGTTCGG-3'

  1. “N” represent mixed nucleotides added to accommodate strain differences in the msp1 gene. Nucleotides in bold face are Illumina adapter overhangs while those in normal face are gene specific primers
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Malaria Journal

ISSN: 1475-2875

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