Table 2 Summary of laboratory bioassay methodology and outcome measures for assessing the insecticidal durability of Interceptor® G2 and Royal Guard® in Benin compared to Interceptor®
ITN type | Brand name | Active ingredient | Mosquito strain | Mosquito age and status (N) | Primary test method (exposure time) | Key outcome measures | ITN efficacy criteria | Remarks/additional tests |
|---|---|---|---|---|---|---|---|---|
Pyrethroid-only | Interceptor | alpha-cypermethrin | Susceptible An. gambiae s.s. Kisumu | unfed, 3–5 days old (40–50/net) | cone bioassays (3 min) | Knockdown, 24 h Mortality | knock-down ≥ 95% or mortality ≥ 80% | Tunnels for failed nets |
Pyrethroid-chlorfenapyr | Interceptor G2a | alpha-cypermethrin | not tested | not tested | not tested | not tested | not tested | not tested due to lower dose of pyrethroid |
chlorfenapyr | Pyrethroid-resistant An. coluzzii Akron | unfed, 5–8 days old (100/net) | Tunnel tests (overnight) | 72 h Mortality and Blood-feeding inhibition | Mortality ≥ 80% or Blood-feeding inhibition ≥ 80% | 1 net piece per ITN | ||
Pyrethroid- pyriproxyfen | Royal Guard | alpha-cypermethrin | Susceptible An. gambiae s.s. Kisumu | unfed, 5–8 days old (40–50/net) | cone bioassays (3 min) | Knockdown, Mortality | knock-down ≥ 95% or mortality ≥ 80% | Tunnels for failed nets |
pyriproxyfen | Pyrethroid-resistant An. coluzzii Akron | blood-fed, 5–8 days old (80–100/net) | cone bioassays (3 min) plus ovary dissection after 72 h | % reduction in fertility relative to controlb | Reduction in fertility relative control | > 30% fertility required in control |