Table 2 Performance of microscope in diagnosis of malaria compared to nPCR, Arba Minch Zuria district, Southwest Ethiopia (November 2019–Janurary 2020)
Plasmodium species | PCR | Total | Sensitivity (95% CI) | Specificity (95% CI) | PPV (95%CI) | NPV (95% CI) | Percentage agreement (%) | Kappa value | |
|---|---|---|---|---|---|---|---|---|---|
+ve | −ve | ||||||||
Microscopy vs. PCR | |||||||||
Overall | |||||||||
+ve | 191 | 2 | 193 | 96.5 (94.7–98.3) | 99.2 (98.4–100) | 99.0 (98.1–99.9) | 97.2 (95.6–98.8) | 97.9 | 0.959 |
−ve | 7 | 247 | 254 | ||||||
P. falciparum | |||||||||
+ve | 75 | 14 | 89 | 89.3 (86.2–92.8) | 96.1 (94.3–97.9) | 84.3 (80.9–87.7) | 97.5 (96.0–97.0) | 94.8 | 0.834 |
−ve | 9 | 349 | 358 | ||||||
P. vivax | |||||||||
+ve | 93 | 8 | 101 | 91.2 (88.6–93.8) | 97.7 (96.3–99.1) | 92.1 (89.6–94.6) | 97.4 (95.9–98.9) | 96.2 | 0.89 |
−ve | 9 | 337 | 346 | ||||||
Mixed infection | |||||||||
+ve | 1 | 2 | 3 | 8.3 (5.7–10.9) | 99.5 (98.8–100) | 33.3 (28.9–37.7) | 97.5 (96.0–99.0) | 12.4 | 0.03 |
−ve | 11 | 433 | 444 | ||||||