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Table 1 Plasmodium falciparum-specific peaks found by MALDI-TOF analysis

From: Detection and quantification of Plasmodium falciparum in human blood by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a proof of concept study

Sample type

Culture type

3D7

HB3

IT4

NC

3D7

NC

3D7

NC

3D7

NC

3D7

NC

3D7

NC

% Culture

100

100

100

100

50

50

25

25

12,5

12,5

7,5

7,5

4

4

% EDTA dilution

0

0

0

0

50

50

75

75

87,5

87,5

92,5

92,5

96

96

Microscopy results

Ring stages

54

49

30

0

32

0

13

0

7

0

4

0

2

0

Late stages

8

9

9

0

6

0

3

0

1

0

0

0

0

0

Total IRBC

3,1%

2.9%

2.5%

None

1,9%

None

0,80%

None

0,40%

None

0,20%

None

0,10%

None

MALDI-TOF results

Successful runs

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

3 of 3

2 of 3

3 of 3

3 of 3

3 of 3

MALDI-TOF diagnose

Pos

Pos

Pos

Neg

Pos

Neg

Pos

Neg

Pos

Neg

Pos

Neg

Pos

Neg

Peaks found (m/z)

1014

+

+

+

–

+

–

+

–

–

–

–

–

–

–

1130

+

+

+

–

+

–

+

–

+

–

+

–

+

–

1366

+

+

+

–

+

–

+

–

+

–

+

–

+

–

1470

+

+

+

–

+

–

–

–

–

–

–

–

–

–

1657

+

+

+

–

+

–

+

–

+

–

+

–

+

–

1984

+

+

+

–

+

–

+

–

–

–

–

–

–

–

2006

+

+

+

–

+

–

+

–

+

–

+

–

+

–

2473

+

+

+

–

+

–

+

–

+

–

+

–

+

–

2527

+

+

+

–

+

–

+

–

–

–

–

–

–

–

3176

+

–

–

–

+

–

+

–

–

–

–

–

–

–

4042

+

+

+

–

+

–

+

–

+

–

–

–

–

–

4300

+

–

–

–

+

–

–

–

–

–

–

–

–

–

4332

+

+

+

–

+

–

+

–

+

–

+

–

–

–

4421

–

+

–

–

–

–

–

–

–

–

–

–

–

–

4432

+

–

–

–

+

–

+

–

+

–

+

–

+

–

4704

+

–

–

–

+

–

+

–

+

–

+

–

–

–

4765

+

–

+

–

–

–

–

–

–

–

–

–

–

–

4773

–

+

–

–

–

–

–

–

–

–

–

–

–

–

4957

+

+

+

–

+

–

+

–

–

–

–

–

–

–

5547

+

+

+

–

+

–

–

–

–

–

–

–

–

–

5567

+

+

+

–

+

–

+

–

+

–

+

–

+

–

5705

+

–

–

–

+

–

–

–

–

–

–

–

–

–

6086

+

–

–

–

+

–

+

–

+

–

+

–

+

–

6286

+

–

–

–

+

–

+

–

–

–

–

–

–

–

6500

+

+

+

–

+

–

+

–

+

–

+

–

+

–

6533

+

–

+

–

+

–

+

–

+

–

+

–

–

–

6760

+

+

+

–

+

–

+

–

+

–

+

–

+

–

7886

+

+

+

–

+

–

–

–

–

–

–

–

–

–

8086

+

+

+

–

+

–

+

–

–

–

–

–

–

–

8568

+

+

+

–

+

–

+

–

+

–

+

–

+

–

8843

–

+

–

–

–

–

–

–

–

–

–

–

–

–

9340

–

–

+

–

–

–

–

–

–

–

–

–

–

–

9409

+

–

–

–

+

–

+

–

–

–

–

–

–

–

9530

+

–

+

–

+

–

–

–

–

–

–

–

–

–

9546

+

+

–

–

–

–

–

–

–

–

–

–

–

–

12,993

+

+

+

–

+

–

+

–

+

–

+

–

+

–

  1. Plasmodium falciparum-specific peaks found by MALDI-TOF analysis using different parasite strains and dilutions. + indicates the presence of a peak at the given m/z. – indicates the absence of a peak at the given m/z. 3D7, HB3 and IT4 indicate the strains of P. falciparum used in the experiment. NC = Negative control cultures consisting of uninfected erythrocytes. IRBC = Plasmodium-infected red blood cells. EDTA dilution = fresh type 0 blood in EDTA drawn from healthy subjects. Giemsa-Stained blood smears were used for microscopy. A total of 2000 red blood cells were reviewed for microscopy of each P. falciparum culture. A total of 100 microscopic fields were reviewed for negative control cultures. Mass spectra were compared by visual inspection using the FlexAnalysis software (Bruker Daltonics, Bremen, Germany) see method section for details. Plasmodium cultures and negative controls were prepared using identical steps. See method section for details
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Malaria Journal

ISSN: 1475-2875

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