Standardization of DNA extraction from paraffinized spleen samples: molecular diagnosis of human malaria

Malaria Journal

Table 1 Primers and probes used for amplification via qPCR.

Assay	Primers	Sequence 5′–3′
QMAL	Forward	TTA GAT TGC TTC CTT CAG TRC CTT ATG
	Reverse	TGT TGA GTC AAA TTA AGC CGC AA
	Probe	6FAM—TCA ATT CTT TTA ACT TTC TCG CTT GCG CGA—BHQ1
PvmtCOX1	PvMt_cox1_Fw	TTATATCCACCATTAAGTACATCACTT
	PvMt_cox1_Rev	AACCTTTAGATCTTAGATGCATTACA
	PvMt_cox1_Probe	FAM-CCTGTTGCAGTAGATGTTATCATTG-BHQ1
cfDNA	For	GGC ACA CGT GGC TTT TCG
	Rev	GGT GAA CCT CGT AAG TTT ATG CAA
	Probe	VIC5-TCA GGA CGT CGA GTG GAC ACG GTG-3 TAMRA

ISSN: 1475-2875