Standardization of DNA extraction from paraffinized spleen samples: molecular diagnosis of human malaria

Malaria Journal

Table 2 Results of the steps of deparaffinization, weight, DNA concentration and qPCR with copy number of tissue sections

Slices/thickness	Wash xylol	Wash—absolute alcohol	Wash—alcohol 70%	Dry weight (mg)	DNA (Blood Kit), ng/µL	DNA (Tissue Kit), ng/µL	PCR Plasmodium sp	PCR Plasmodium vivax	Copy number Plasmodium 1 (copies/µL)	Copy number Plasmodium 2 (copies/µL)	Copy number P. vivax 1 (copies/µL)	Copy number P. vivax 2 (copies/µL)
5/10 µm	One 1′	One 1′	One 1′	70	5	8	Negative	Negative	0	0	0	0
10/10 µm	One 1′	One 1′	One 1′	84.8	9	7	Negative	Negative	0	0	0	0
15/10 µm	One 1′	One 1′	One 1′	125	2	15	Negative	Negative	0	0	0	0
20/10 µm	One 1′	One 1′	One 1′	122.3	15	20	Negative	Negative	0	0	0	0
25/10 µm	One 1′	One 1′		177	47	32	Negative	Negative	0	0	0	0
6/10 µm	Two 1′	Two 1′		62	61.8	70	Negative	Negative	0	0	0	0
8/10 µm	Two 1′	Two 1′		41	35	41	Negative	Negative	0	0	0	0
5/20 µm	Two 1′	Two 1′		23	40	32	Negative	Negative	0	0	0	0
10/20 µm	Two 1′	Two 1′		40	50	55	Positive	Positive	75.01 [73.8–76.4]	86.03 [84.2–87.9]	89.2 [87.4–91.1]	106.06 [105.2–108]

DNA concentration obtained per ng/µL (DNA). Number of samples selected in qPCR for samples taken with the sample collection kit (copy number Plasmodium 1), number of copies obtained via qPCR for samples using the tissue kit (copy number Plasmodium 2), number of samples obtained via qPCR for samples using blood kit (copy number P. vivax 1), number of copies obtained via qPCR for samples using the tissue kit (copy number P. vivax). Confidence interval shown in square brackets [CI]

ISSN: 1475-2875