Fig. 3

HumAb Synergy Experiments: A, C The first column shows growth inhibition curves for humAbs 065098, and 094083 individually, with and without the presence of 099100; 099100 was held constant at its IC₂₅ (50 µg/mL) at each concentration (i.e., 800 µg/mL 065098 + 50 µg/mL 099100, 400 µg/mL 065098 + 50 µg/mL 099100, etc.); the green dot depicts the percent inhibition of 099100 at its IC₂₅ (50 µg/mL); All points are mean and standard deviation of triplicate culture. The table below the figures shows differences in IC₅₀ of 065098 and 094083 with and without 099100. B, D Assessment by SynergyFinder web-based application software to visualize multi-antibody multi dose combination data based on the independent model Bliss: 3-dimensional representation of the dose–response matrices showing varying concentrations of 065098 B and 094083 D on the x-axis, varying concentrations of 099100 on the y-axis (using data from Fig. 2), and synergy score (δ) on the z-axis; each matrix is colour coded to show synergy distribution, a corresponding Bliss synergy score (BSS), and topography (peaks/valleys) at specific concentrations; green/valleys denotes antagonism, red/peaks denotes additivity or synergy. Scale for BSS: score < 0 = antagonism, 10 < score > 0 = additivity, score > 9.9 = synergy