Table 1 Experimental groups and immunization protocol
Group no | Antigen/Adjuvant(s) | Ag (μg/mouse) | Adjuvants (μg/mouse) | ||||
|---|---|---|---|---|---|---|---|
Prime* | Boost1** | Boost 2*** | CpG | MPL | QS-21 | ||
1 (n = 9) | cd-HAP/− | 10 | 5 | 5 | – | – | – |
2 (n = 9) | cd-HAP/CpG | 10 | 5 | 5 | 10 | – | – |
3 (n = 9) | cd-HAP/MPL | 10 | 5 | 5 | – | 10 | – |
4 (n = 9) | cd-HAP/QS-21 | 10 | 5 | 5 | – | – | 10 |
5 (n = 9) | cd-HAP/CpG + MPL + QS-21 | 10 | 5 | 5 | 5 | 5 | 5 |
6 (n = 9) | −/1 × PBS | – | – | – |  |  |  |
7 (n = 9) | −/CpG | – | – | – | 10 | – | – |
8 (n = 9) | −/MPL | – | – | – | – | 10 | – |
9 (n = 9) | −/QS-21 | – | – | – | – | – | 10 |
10 (n = 9) | −/CpG + MPL + QS-21 | – | – | – | 5 | 5 | 5 |