Table 1 Primers and probes used for detection of Plasmodium species in pregnancy, northwest Ethiopia, 2022
Target Gene | Sequence | Fluorophores |
|---|---|---|
Pspp18S F | GCT CTT TCT TGA TTT CTT GGA TG | – |
Pspp18S R | AGC AGG TTA AGA TCT CG TTC G | – |
Pspp18S Cy5 | ATG GCC GTT TTT AGT TCG TG | Cy5-BHQ2 |
HsRNaseP F | AGA TTT GGA CCT GCG AGC G | – |
HsRNaseP R | GAG CGG CTG TCT CCA CAA GT | – |
HsRNaseP YYE | TTC TGA CCT GAA GGC TCT GCG CG | YYE-BHQ1 |
PfvarATS F | CCCATACACAACCAAYTGGA | – |
PfvarATS R | TTCGCACATATCTCTATGTCTATCT | – |
PfvarATS FAM | TRTTCCATAAATGGT | Fluorescein |
Pv18S F | ACTAGGCTTTGGATGAAAGATTTTA | – |
Pspp18S R | AACCCAAAGACTTTGATTTCTCATAA | – |
Pv18S probe | GAATTTTCTCTTCGGAGTTTAT | Cy5-BHQ2 |