Assessment of Plasmodium falciparum drug resistance associated molecular markers in Mandla, Madhya Pradesh, India

Table 1 Primer sequence and PCR condition used for amplification of P. falciparum drug resistance genes

Gene	Primer name	Primer sequence	PCR product length (bp)	Denaturation	Annealing	Elongation	No of cycles
Pfmdr1 primary	MDR1	AGAGTACCGCTGAATTATTTAG	1472	94 °C, 1 min	53 °C, 1 min	72 °C, 1 min	35
	MDR2	TTCATTTGATGTCATAGAATTCG
Pfmdr1 nested	MDR3	ATGTTTACCTGCACAACATAGAA	856	94 °C, 1 min	52 °C, 1 min	72 °C, 1 min	30
	MDR4	CATAAACATACTAATAAGTACACC
Pfdhfr primary	PF1	TTTATATTTTCTCCTTTTTA	718	94 °C, 1 min	45 °C, 45 s	72 °C, 1 min	35
	PR1	CATTTTATTATTCGTTTTCT
Pfdhfr nested	PF1	TTTATATTTTCTCCTTTTTA	648	94 °C, 1 min	45 °C, 45 s	72 °C, 1 min	30
	NR2	ACAGAAATAATTTGATACTCA
Pfdhfs primary	F1	CCATTCCTCATGTGTATACAACAC	1167	94 °C, 1 min	55 °C, 1 min.	72 °C, 1 min. 30 s	35
	R1	CTTGGTCTATTTTTGTTAAAACATCC
Pfdhfs nested	F2	TGGAATATTAAATGTTAATTATGA	735	94 °C, 1 min	50 °C, 45 s	72 °C, 1 min	30
	R2	TTTTCATTTTGTTGTTCATCATGT
PfK 13	K13_F	GCCAAGCTGCCATTCATTTG	849	94 °C, 45 s	60 °C, 1 min	72 °C, 1 min 30 s	35
	K13_R	GCCTTGTTGAAAGAAGCAGA

Pfmdr1: Plasmodium falciparum multi drug resistance gene1; Pfdhfr: Plasmodium falciparum dihydrofolate reductase, Pfdhps: Plasmodium falciparum dihydropteroate synthase; Pfk13: Plasmodium falciparum kelch13, °C: temperature in degree Celsius; min: minute; s: seconds; bp: base pair; n = number; %= percentage

ISSN: 1475-2875