Fig. 4
Effect of PBANKA_0519900 deletion on the asexual and sexual development of P. berghei ANKA. Female B6 mice were infected with 1 × 104 erythrocytes parasitized with P. berghei ANKA with PBANKA_0519900 deletion (Δ0519900). As a control, P. berghei ANKA with p230 deletion was inoculated intravenously into mice. *Significant difference compared to controls. A Time course of parasitaemia. Results are expressed as mean ± SD from four mice. Experiments using four mice were performed in triplicate. B Survival rate. Data are the mean ± SD from four mice and are representative of three independent experiments. C, D Percentages of mature female (C) and male (D) gametocytes. Erythrocytes parasitized with Plasmodium were incubated for 28 h. The percentages of male and female gametocytes were calculated as follows: ([number of male or female gametocytes] ÷ [total number of schizonts plus male and female gametocytes]) × 100. Results are expressed as the mean ± SD of three independent experiments