Characterization of a nuclear transport factor 2-like domain-containing protein in Plasmodium berghei

Malaria Journal

Table 1 Results of immunoprecipitation coupled with mass spectrometry for 1019700::mCherry parasites

Accession #	Description	Σ# PSMs	# AAs	MW [kDa]	Calc. pI
PBANKA_1019700	Conserved Plasmodium protein, unknown function	1098	1176	136.8	8.50
PBANKA_0519900	Conserved Plasmodium protein, unknown function	212	1591	180.2	9.25
PBANKA_1359300	VPS13 domain-containing protein, putative	209	5434	629.9	7.64
PBANKA_0415500	Coatomer subunit beta, putative	188	999	116.5	5.90
PBANKA_0210600	Ubiquitin carboxyl-terminal hydrolase, putative	181	2788	326.0	8.48
PBANKA_1012800	Voltage-dependent anion-selective channel protein, putative	175	289	33.3	9.17
PBANKA_0207000	Calcium-transporting ATPase, putative	172	1120	127.3	7.53
PBANKA_0823000	Ubiquitin-like protein, putative	165	1384	161.4	8.66
PBANKA_1438300	Phospholipid-transporting ATPase, putative	164	1603	187.7	8.76
PBANKA_0316200	Plasmodium exported protein, unknown function	161	1739	206.1	3.97
PBANKA_1310800	40S ribosomal protein S5, putative	158	271	29.9	10.05
PBANKA_0802300	HECT-type E3 ubiquitin ligase UT, putative	150	3439	405.1	7.91

Proteins were extracted from 1019700::mCherry schizont- and gametocyte-enriched cultures after 22 h of culturing. Proteins with 50 peptide spectral matches (averaged across three independent experiments) and a fold change ≥ 5 compared to controls are listed. Control experiments involved immunoprecipitation of wild-type P. berghei ANKA using anti-mCherry beads coupled to mass spectrometry. Experiments were performed in triplicate. The results presented are sums from three independent experiments
PSMs peptide spectral matches, AAs amino acids, MW molecular weight, Calc. pI calculated isoelectric point

ISSN: 1475-2875