Fig. 1
From: Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing
Specificity of the pfs230 genotyping marker for detection of mature gametocytes. Lane A in each panel represents RT-PCR performed on RNA extracted from a high parasite density culture (100,000 parasites/µL) of a non-gametocyte-producing P. falciparum strain MRA-1183, with corresponding RT negative control (AN). Lane G represents the same RT-PCRs on RNA extracted from NF54 gametocytes (10 gametocytes/µL) with corresponding RT negative (GN) control. Lane N and P represent the PCR negative and positive controls, respectively. Lane L represents a 100bp DNA ladder