Fig. 2
From: Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing
Agreement between expected clone abundance (gametocyte mixture ratios) and observed abundance (clone frequency as estimated by the proportion of reads corresponding to a given clone) of RNA and DNA extracted from NF54 and MLW5 mature gametocyte mixtures. A Stacked bar graphs depicting observed clone frequency after amplicon sequencing of mature gametocyte mixtures of NF54 (N) and MLW5 (M) strains DNA (D1 and D2 are replicates) and RNA (R1 and R2 are replicates) were mixed in the following ratios: 1:0, 10:1, 5:1, 2:1, 1:1, and the reverse. B, C The observed RNA and DNA clone frequencies for each clone are plotted against the expected frequencies (black diagonal line) and were found to be highly concordant (RNA CCC = 0.97; DNA CCC = 0.92)