Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human Equilibrative Nucleoside Transporter and the human Facilitative Nucleobase Transporter
© Quashie et al; licensee BioMed Central Ltd. 2010
Received: 29 October 2009
Accepted: 29 January 2010
Published: 29 January 2010
Plasmodium parasites are unable to synthesize purines de novo and have to salvage them from the host. Due to this limitation in the parasite, purine transporters have been an area of focus in the search for anti-malarial drugs. Although the uptake of purines through the human equilibrative nucleoside transporter (hENT1), the human facilitative nucleobase transporter (hFNT1) and the parasite-induced new permeation pathway (NPP) has been studied, no information appears to exist on the relative contribution of these three transporters to the uptake of adenosine and hypoxanthine. Using the appropriate transporter inhibitors, the role of each of these salvage pathways to the overall purine transport in intraerythrocytic Plasmodium falciparum was systematically investigated.
The transport of adenosine, hypoxanthine and adenine into uninfected and P. falciparum-infected human erythrocytes was investigated in the presence or absence of classical inhibitors of the hFNT1, hENT1 and NPP. The effective inhibition of the various transporters by the classical inhibitors was verified using appropriate known substrates. The ability of high concentration of unlabelled substrates to saturate these transporters was also studied.
Transport of exogenous purine into infected or uninfected erythrocytes occurred primarily through saturable transporters rather than through the NPP. Hypoxanthine and adenine appeared to enter erythrocytes mainly through the hFNT1 nucleobase transporter whereas adenosine entered predominantly through the hENT1 nucleoside transporter. The rate of purine uptake was approximately doubled in infected cells compared to uninfected erythrocytes. In addition, it was found that the rate of adenosine uptake was considerably higher than the rate of hypoxanthine uptake in infected human red blood cells (RBC). It was also demonstrated that furosemide inhibited the transport of purine bases through hFNT1.
Collectively, the data obtained in this study clearly show that the endogenous host erythrocyte transporters hENT1 and hFNT1, rather than the NPP, are the major route of entry of purine into parasitized RBC. Inhibitors of hENT1 and hFNT1, as well as the NPP, should be considered in the development of anti-malarials targeted to purine transport.
Since purine salvage from the host milieu is an important physiological requirement for growth and multiplication of Plasmodium falciparum, purine transporters are regarded as ideal targets for the development of novel purine-based anti-malarial drugs to combat malaria [1–3].
Non-infected human erythrocytes take up nucleosides through the human equilibrative nucleoside transporter hENT1  and purine bases through the facilitative nucleobase transporter hFNT1 [5, 6]. hENT1, but not hFNT1, is potently inhibited by 6-[(nitrobenzyl)-thio]9-β-d-ribofuranosylpurine (NBMPR) . Yet, while NBMPR completely blocks adenosine uptake in human erythrocytes, infection with P. falciparum induced an additional, NBMPR-insensitive, uptake mechanism in these cells [2, 8] and this mechanism does not distinguish between the d and l-enantiomers of adenosine .
These observations helped define the concept of the new permeation pathway (NPP), an apparently non-saturable channel-like system that transports low molecular weight compounds including purines, and is formed after parasite invasion of erythrocytes . Several researchers have described the NPP as exhibiting functional characteristics of an anion channel; being selective for Cl- over K+ and blocked by a range of classical anion channel inhibitors including furosemide and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) [11–13]. Using the patch-clamp technique, two groups confirmed the presence and properties of the NPP in P. falciparum-infected erythrocytes [14, 15].
While it is clearly established that the NPP is capable of mediating adenosine uptake, it is less clear whether this function is important in terms of its contribution to the overall purine salvage given the continued presence of both hENT1 and hFNT1 in parasitized RBC. It is also unknown whether hypoxanthine, the preferred purine of P. falciparum[16, 17], is a permeant of the NPP. In the current study we thus investigated the degree to which the overall purine salvage by intraerythrocytic P. falciparum is dependent on the NPP. The transport of adenosine, hypoxanthine and adenine into P. falciparum-infected and uninfected human erythrocytes was, therefore, studied in the presence of selective inhibitors. The results show that despite the presence of the NPP, transport of nucleoside and nucleobase into infected RBC is largely through hENT1 and hFNT1, respectively.
Transport assays with infected cells were performed with the standard 3D7 drug-sensitive laboratory clone of P. falciparum, originally obtained from David Walliker (School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK). Human blood and serum used for Plasmodium culture were obtained from the Glasgow and West of Scotland Blood Transfusion Service. Asexual parasites of P. falciparum were maintained in continuous culture using slightly modified standard methods . Briefly, parasites were grown in RPMI 1640 medium supplemented with 5.94 g/l HEPES, 0.21% NaHCO3 and 10% heat-inactivated normal human serum at 5% haematocrit. The culture was incubated at 37°C under a gas mixture of 1% O2, 3% CO2 and 96% N2, and medium was changed daily. Prior to use in experiments, parasite cultures were synchronized using a previously described method .
Using the method described by the group of Kramer , synchronized cultures of parasitized erythrocytes at trophozoite stage were enriched for infected cells on isotonic Percoll™ (Amersham Biosciences) gradients (density 1.090 and osmolality 320 mosm (kg H2O)-1), resulting in a suspension with >80% parasitaemia. The trophozoite-enriched red blood cells harvested were washed three times with Erythrocyte Medium (140 mM NaCl, 5 mM KCl, 20 mM Tris/HCl (pH 7.4), 2 mM MgCl2, and 0.1 mM EDTA) and re-suspended in the same media for all the experiments performed in this study. In experiments that compared uptake into uninfected erythrocytes with parasitized erythrocytes, erythrocytes from the same donor were incubated in parallel with the infected erythrocyte cultures under the same conditions and were similarly centrifuged through Percoll before being used in the assay. Cell concentrations were determined with an improved Neubauer counting chamber.
All experiments with P. falciparum parasites were carried out using synchronized and Percoll enriched trophozoite-infected human erythrocytes. [2,8-3H]-Adenine (27 Ci/mmol) used in this study was purchased from Moravek (USA) whilst [2,8-3H]-adenosine (23 Ci/mmol) and [8-3H]-hypoxanthine (19 Ci/mmol) were obtained from Amersham Pharmacia Biotech (UK). Unlabelled adenine, hypoxanthine, adenosine and uridine of highest purity were obtained from Sigma. Transport of [3H]-adenosine, [3H]-adenine and [3H]-hypoxanthine into P. falciparum-infected erythrocytes and uninfected erythrocytes in the presence of NBMPR (Sigma), furosemide (Sigma), NPPB (Sigma) or 1 mM unlabelled permeant was determined exactly as previously described . Briefly, equal volumes of a suspension of the cells (usually at a concentration of 4 × 107 cells/assay) and a radiolabeled permeant at twice its final concentration were mixed for predetermined times. Influx of extracellular radiolabeled permeant into the cells was terminated by the addition of 1 ml ice-cold stop solution followed by a quick spin at 13,000 × g to sediment cells through an oil-mix (300 μl of 5 parts dibutylphthalate (Aldrich): 4 parts dioctyl phthalate (Aldrich), v/v). The stop solutions used in the experiments in this study contain dilazep, furosemide, or papaverine (all from Sigma), to terminate uptake through hENT1, NPP and hFNT1, respectively. Cold stop solutions are designed to stop further uptake of permeant into the cells while the cells pellet below the oil-mix . All experiments described in this study were performed in triplicate.
After the uptake of the permeant, the pellets were retrieved and processed for scintillation counting of radiolabel incorporated using the method previously described by Saliba and colleagues . All uptake data presented in this study are presumed to represent a "mediated uptake," defined as the total uptake of the permeant apart from simple diffusion through the plasma membrane, which is not a saturable process. Non-mediated uptake of the respective permeant was assessed by determining the rate of uptake of the radiolabeled permeant in the presence of 1 mM unlabelled permeant as well as with cells and permeant at 0°C and subtracted from total uptake. The rate of uptake of the permeant was obtained from a plot of permeant concentration against time using the Prism 4 (GraphPad) software package. Ki values were calculated from the equation Ki = IC50/(1+ [L/Km]), where L is the permeant concentration. The Km and Vmax values were obtained for the transporters using the Michaelis-Menten plot.
Finally, it was tested whether adenine competitively inhibits [3H]-hypoxanthine in infected RBC. In both uninfected and P. falciparum-infected erythrocytes, hypoxanthine uptake was completely blocked in the presence of 1 mM adenine. It is thus concluded that hypoxanthine, like adenosine, is overwhelmingly taken up through hFNT1 rather than NPP.
Purines, and in particular hypoxanthine, are essential nutrients that the intracellular malaria parasite requires for growth and multiplication [17, 29]. Since parasites are only able to obtain these nutrients from the host milieu through salvage, availability of detailed information of the transporters involved in their uptake is crucial for the development of purine-based anti-malarial drugs. Much progress has been made in the last few years in identifying and characterizing purine transporters on the P. falciparum cell surface [1, 30, 31], and at least one of these transporters seems to be essential for growth at physiological purine concentrations .
The data presented in this manuscript clearly show that hypoxanthine enters P. falciparum-infected human erythrocytes overwhelmingly through a saturable, adenine-sensitive transport mechanism, which is compatible with the human FNT1 nucleobase transporter, but not with the properties reported for NPP [10, 11]. Adenosine similarly enters predominantly through a saturable transporter, consistent with hENT1. The presence of these transporters on the plasma membrane of non-infected RBC has previously been reported . Given the complexity of nutrient transport in the human erythrocyte, especially after Plasmodium invasion, it is imperative that a systematic approach be employed in order to obtain a genuine conclusion. Therefore, using classical transport inhibitors such as NPPB, furosemide and NBMPR, non-metabolized substrates such as uridine, and competitive inhibitors such as adenine for hypoxanthine uptake, the various pathways involved in purine uptake into P. falciparum-infected RBC were systematically dissected.
Prior to their use, the selectivity and IC50 values of these inhibitors was re-assessed to confirm which transporters are blocked and at what concentration. Uptake of sorbitol, a well-known substrate of the NPP [11, 23], into infected RBC was almost fully blocked by NPPB. This uptake could not have occurred through any of the endogenous transporters, as there was no sorbitol uptake in uninfected erythrocytes. Similarly, it was verified whether known NPP inhibitors affected transport through the endogenous nucleoside and nucleobase transporters. NPPB did not affect either carrier, whereas furosemide was a good inhibitor of hFNT1, but not of hENT1. Although the mechanism for the furosemide effect on hFNT1 was not further investigated, the finding is potentially important, particularly towards efforts in search of purine-based antiplasmodial compounds, as similar concentrations of furosemide would inhibit both the NPP and hFNT1. The furosemide inhibition of [3H]-hypoxanthine and [3H]-adenine uptake reported here was clearly not through inhibition of the NPP as it was equally observed in infected and uninfected RBC. In view of the above inhibitor profile, it is evident that purine uptake in infected erythrocytes, being saturable and NPPB-insensitive, is mostly mediated by the human transporters hENT1 and hFNT1.
There is no doubt that nucleosides and nucleobases can enter the infected RBC through the furosemide/NPPB-sensitive new permeation pathways (NPP). For example, Gero and co-workers showed residual uptake of d-adenosine in infected human erythrocytes in the presence of the hENT1 inhibitor NBMPR , estimated at 20% of the uninhibited uptake rate , although this was not observed in the present study (Figure 2A). Kirk and colleagues  similarly demonstrated the NBMPR-insensitive, but partly furosemide and NPPB-sensitive, influx of adenosine and thymidine. However, that study used a concentration of 1 mM extracellular nucleoside, whereas the present study used a more physiological concentration of 0.5 μM. Whilst the higher concentrations were useful to demonstrate that nucleoside uptake through NPP is possible, the present investigation has sought to address the question whether the intracellular parasite is dependent on the NPP for purine uptake at physiological concentrations, which range from 0.1 - 1 μM for both adenosine and hypoxanthine [33, 34]. Hypoxanthine uptake in P. falciparum-infected RBC was recently demonstrated, at 150 μM, to be ~50% inhibited by dantrolene, a newly discovered inhibitor of NPP, but the effect of dantrolene on hFNT1-mediated transport was not tested . As it is here reported that furosemide also inhibits both the hFNT1 and NPP transport pathways, the possibility of similar action by dantrolene should not be discounted.
Notwithstanding the above, some of the reports from the group of Gero reported the NBMPR-insensitive uptake of d-adenosine at 1 μM [8, 36]. They further showed uptake of l-adenosine and l-thymidine by P. falciparum-infected human erythrocytes, although these non-physiological enantiomers were not substrates for hENT1 [9, 36]. Instead, uptake of l-nucleosides uptake was non-saturable and sensitive to furosemide  and NPPB , indicative of NPP. The potential of the NPP to take up low levels of purines, perhaps indiscriminately, may be of pharmacological importance, allowing the entry of toxic enantiomers and analogues selectively into the infected erythrocytes only.
Gero and colleagues also observed  that nucleoside transport inhibitors, including NBMPR, dilazep and dipyridamole all display intrinsic activity against P. falciparum in vitro, at concentrations that inhibit hENT1 . This appears to show that inhibition of hENT1 leads to purine starvation of the developing parasite, which would be consistent with the observation that the rate of adenosine uptake is considerably higher than the rate of hypoxanthine uptake in infected RBC. Hypoxanthine is the preferred purine source for P. falciparum[17, 29], but since adenosine is rapidly converted to hypoxanthine in the cytosol of the infected erythrocyte , it may be that inhibitors of hENT1 should have at least a growth-delaying effect on P. falciparum in vivo and that a combination with an inhibitor of hFNT1 would be lethal. It must be emphasized, however, that such a strategy would also deprive the uninfected erythrocytes of a purine source and that, while erythrocytes do not need purines for nucleic acid synthesis, their energy balance would be affected. However, it is likely that infected erythrocytes will be far more severely affected as their intracellular purine stores would be rapidly depleted by the highly efficient P. falciparum purine salvage system . Yet, it is not certain that the above hENT1 inhibitors perform their anti-malarial activity by blocking the human adenosine transporter. For instance, Carter et al report that the main P. falciparum nucleoside transporter, PfNT1, is 85% inhibited by 10 μM dipyridamole, although Parker et al in a similar study did not find PfNT1 sensitive to dipyridamole. Furthermore, Gero and colleagues present clear evidence that NBMPR, in particular, is internalized and metabolized by the parasites . Thus, while not transported by hENT1, the transport inhibitors could enter the Plasmodium-infected cells through NPP, and either inhibit the parasite's own purine transporters on its plasma membrane, or attack an intracellular target.
The data presented in the current manuscript show that uptake of adenosine in infected erythrocytes was almost completely inhibited by NBMPR and fully saturable - features which are inconsistent with the properties of the NPP. It may therefore be concluded that the NPP plays only a minor role in the salvage of this nucleoside in infected RBC. The observation that the rate of adenosine uptake in infected RBC was identical in the presence or absence of 100 μM of the NPP-inhibitor furosemide further supports this conclusion. Owing to the high rate of adenosine transport in erythrocytes, the equilibrative nature of hENT1, and the rapid metabolism of adenosine inside both infected and non-infected RBC, it is extremely difficult to measure true initial rates of [3H]-adenosine transport accurately in this system , and the data presented here refer to uptake, being the sum of transport and metabolism, rather than transport. However, the authors believe the conclusion that influx of adenosine is mediated by hENT1 rather than NPP is completely justified. To further validate these observations, the experiment was repeated using uridine as permeant, which is a substrate of hENT1, but not metabolized by human erythrocytes  or salvaged by P. falciparum. The results show that, like adenosine, transport of uridine (25 μM) is overwhelmingly mediated by hENT1, being saturable and not inhibitable by NPPB.
This study clearly demonstrated that uptake of the nucleobases in infected human erythrocytes is also mainly through the exogenous hFNT1 transporter despite the presence of the NPP. As reported by Domin and colleagues, the non-infected human erythrocyte readily takes up adenine and hypoxanthine by facilitated diffusion through hFNT1 and, using ice-cold papaverine to instantly stop transport, it is possible to measure initial rates of transport over a brief but sufficiently long period . In Figure 5A, the highest level of hypoxanthine uptake corresponds to an intracellular concentration of 0.39 μM, using the estimated volume of 75 fL for infected human erythrocytes reported by Saliba and co-workers , compared to an extracellular concentration of 1 μM and had thus not reached equilibrium. The uptake was clearly saturable and the response to NPPB was variable, resulting in a small increase in the uptake rate (Figure 5A) or, no difference, or a minor inhibition (Figure 5B). In addition, the study shows that initial rates of transport were completely inhibited by adenine (Figure 6), which cannot be explained either in terms of [3H]-hypoxanthine transport through NPP, or through effects on hypoxanthine metabolism: adenine does not intersect with hypoxanthine metabolism either in the erythrocyte or inside the parasite [37, 39].
An interesting observation made in the current study is the apparent increase in the uptake rate of these nucleosides into infected RBC compared to uninfected erythrocytes. This was observed consistently for hypoxanthine, adenosine and uridine. While it is to be expected that infected RBC will take up more purines due to greater demand for nucleic acid synthesis from the intracellular parasite, these observations appear to reflect increased transport capacity, rather than increased usage. This is particularly obvious for uridine, which is not metabolized by Plasmodium. While this phenomenon has been reported previously for other nutrients, including tryptophan and choline (reviewed by Kirk and colleagues) , this had not yet been reported for purine transport across the infected erythrocyte membrane. The mechanism by which Plasmodium species increase nutrient uptake by host carriers is still a subject of intense debate.
Taken together data presented in this study show that nucleobases and nucleosides present at low levels in the host plasma gain entry into the infected erythrocyte predominantly through equilibrative transporters rather than the parasite-induced New Permeation Pathways - a process efficiently driven by the rapid salvage of purines from the erythrocyte cytosol by the intracellular parasite, thus maintaining a concentration gradient across the RBC plasma membrane.
List of abbreviations
human Equilibrative Nucleoside Transporter 1
human Facilitative Nucleobase Transporter 1
New Permeation Pathways
5-nitro-2-(3-phenylpropylamino) benzoic acid
Plasmodium falciparum Nucleoside Transporter 1
red blood cell.
NBQ was supported by a GETfund scholarship from the Ghanaian government and subsequently by a Wellcome Trust Value in People Award. We thank Dr. A. Docherty and the staff at the Glasgow and West of Scotland Blood transfusion services, for the provision of human blood and serum. We are most grateful to Liz Peat of the Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow for technical support.
- Quashie NB, Dorin-Semblat D, Bray PG, Biagini GA, Doerig C, Ranford-Cartwright LC, De Koning HP: A comprehensive model of purine uptake by the malaria parasite Plasmodium falciparum: identification of four purine transport activities in intraerythrocytic parasites. Biochem J. 2008, 411: 287-295. 10.1042/BJ20071460.View ArticlePubMed
- Gero AM, Scott HV, O'Sullivan WJ, Christopherson RI: Antimalarial action of nitrobenzylthioinosine in combination with purine nucleoside antimetabolites. Mol Biochem Parasitol. 1989, 34: 87-97. 10.1016/0166-6851(89)90023-6.View ArticlePubMed
- Downie MJ, Saliba KJ, Broer S, Howitt SM, Kirk K: Purine nucleobase transport in the intraerythrocytic malaria parasite. Int J Parasitol. 2008, 38: 203-209. 10.1016/j.ijpara.2007.07.005.View ArticlePubMed
- Plagemann PG, Wohlhueter RM, Woffendin C: Nucleoside and nucleobase transport in animal cells. Biochim Biophys Acta. 1988, 947: 405-443.View ArticlePubMed
- Domin BA, Mahony WB, Zimmerman TP: Purine nucleobase transport in human erythrocytes. Reinvestigation with a novel "inhibitor-stop" assay. J Biol Chem. 1988, 263: 9276-9284.PubMed
- de Koning H, Diallinas G: Nucleobase transporters (review). Mol Membr Biol. 2000, 17: 75-94. 10.1080/09687680050117101.View ArticlePubMed
- Plagemann PG, Woffendin C: Species differences in sensitivity of nucleoside transport in erythrocytes and cultured cells to inhibition by nitrobenzylthioinosine, dipyridamole, dilazep and lidoflazine. Biochim Biophys Acta. 1988, 969: 1-8. 10.1016/0167-4889(88)90081-X.View ArticlePubMed
- Gero AM, Bugledich EM, Paterson AR, Jamieson GP: Stage-specific alteration of nucleoside membrane permeability and nitrobenzylthioinosine insensitivity in Plasmodium falciparum infected erythrocytes. Mol Biochem Parasitol. 1988, 27: 159-170. 10.1016/0166-6851(88)90035-7.View ArticlePubMed
- Upston JM, Gero AM: Parasite-induced permeation of nucleosides in Plasmodium falciparum malaria. Biochim Biophys Acta. 1995, 1236: 249-258. 10.1016/0005-2736(95)00055-8.View ArticlePubMed
- Kirk K, Tilley L, Ginsburg H: Transport and trafficking in the malaria-infected erythrocyte. Parasitol Today. 1999, 15: 355-357. 10.1016/S0169-4758(99)01495-7.View ArticlePubMed
- Kirk K, Horner HA, Elford BC, Ellory JC, Newbold CI: Transport of diverse substrates into malaria-infected erythrocytes via a pathway showing functional characteristics of a chloride channel. J Biol Chem. 1994, 269: 3339-3347.PubMed
- Kirk K, Horner HA: In search of a selective inhibitor of the induced transport of small solutes in Plasmodium falciparum-infected erythrocytes: effects of arylaminobenzoates. Biochem J. 1995, 311: 761-768.PubMed CentralView ArticlePubMed
- Breuer WV, Kutner S, Sylphen J, Ginsburg H, Cabantchik ZI: Covalent modification of the permeability pathways induced in the human erythrocyte membrane by the malarial parasite Plasmodium falciparum. J Cell Physiol. 1987, 133: 55-63. 10.1002/jcp.1041330107.View ArticlePubMed
- Desai SA, Bezrukov SM, Zimmerberg J: A voltage-dependent channel involved in nutrient uptake by red blood cells infected with the malaria parasite. Nature. 2000, 406: 1001-1005. 10.1038/35023000.View ArticlePubMed
- Huber SM, Uhlemann AC, Gamper NL, Duranton C, Kremsner PG, Lang F: Plasmodium falciparum activates endogenous Cl(-) channels of human erythrocytes by membrane oxidation. EMBO J. 2002, 21: 22-30. 10.1093/emboj/21.1.22.PubMed CentralView ArticlePubMed
- Reyes P, Rathod PK, Sanchez DJ, Mrema JE, Rieckmann KH, Heidrich HG: Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum. Mol Biochem Parasitol. 1982, 5: 275-290. 10.1016/0166-6851(82)90035-4.View ArticlePubMed
- Berman PA, Human L: Hypoxanthine depletion induced by xanthine oxidase inhibits malaria parasite growth in vitro. Adv Exp Med Biol. 1991, 309A: 165-168.View ArticlePubMed
- Trager W, Jensen JB: Human malaria parasites in continuous culture. Science. 1976, 193: 673-675. 10.1126/science.781840.View ArticlePubMed
- Lambros C, Vanderberg JP: Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979, 65: 418-420. 10.2307/3280287.View ArticlePubMed
- Kramer KJ, Kan SC, Siddiqui WA: Concentration of Plasmodium falciparum-infected erythrocytes by density gradient centrifugation in Percoll. J Parasitol. 1982, 68: 336-337. 10.2307/3281203.View ArticlePubMed
- Wallace LJ, Candlish D, de Koning HP: Different substrate recognition motifs of human and trypanosome nucleobase transporters. Selective uptake of purine antimetabolites. J Biol Chem. 2002, 277: 26149-26156. 10.1074/jbc.M202835200.View ArticlePubMed
- Saliba KJ, Horner HA, Kirk K: Transport and metabolism of the essential vitamin pantothenic acid in human erythrocytes infected with the malaria parasite Plasmodium falciparum. J Biol Chem. 1998, 273: 10190-10195. 10.1074/jbc.273.17.10190.View ArticlePubMed
- Ginsburg H, Krugliak M, Eidelman O, Cabantchik ZI: New permeability pathways induced in membranes of Plasmodium falciparum infected erythrocytes. Mol Biochem Parasitol. 1983, 8: 177-190. 10.1016/0166-6851(83)90008-7.View ArticlePubMed
- Staines HM, Ashmore S, Felgate H, Moore J, Powell T, Ellory JC: Solute transport via the new permeability pathways in Plasmodium falciparum-infected human red blood cells is not consistent with a simple single-channel model. Blood. 2006, 108: 3187-3194. 10.1182/blood-2006-02-001693.PubMed CentralView ArticlePubMed
- Plagemann PG, Wohlhueter RM, Erbe J: Facilitated transport of inosine and uridine in cultured mammalian cells is independent of nucleoside phosphorylases. Biochim Biophys Acta. 1981, 640: 448-462. 10.1016/0005-2736(81)90470-3.View ArticlePubMed
- Oliver JM, Paterson AR: Nucleoside transport. I. A mediated process in human erythrocytes. Can J Biochem. 1971, 49: 262-270. 10.1139/v71-481.View ArticlePubMed
- Bungener W, Nielsen G: Nukleinsäurenstoffwechsel bei experimenteller Malaria 2. Einbau von Adenosin und Hypoxanthin in die Nukleinsäuren von Malariaparsiten (Plasmodium berghei und Plasmodium vinckei). Z Tropenmed Parasitol. 1967, 18: 456-462.PubMed
- Fervenza FC, Meredith D, Ellory JC, Hendry BM: Uridine transport in human erythrocytes: data from normal subjects and from patients with renal failure. Exp Physiol. 1991, 76: 53-58.View ArticlePubMed
- Berman PA, Human L, Freese JA: Xanthine oxidase inhibits growth of Plasmodium falciparum in human erythrocytes in vitro. J Clin Invest. 1991, 88: 1848-1855. 10.1172/JCI115506.PubMed CentralView ArticlePubMed
- Parker MD, Hyde RJ, Yao SY, McRobert L, Cass CE, Young JD, McConkey GA, Baldwin SA: Identification of a nucleoside/nucleobase transporter from Plasmodium falciparum, a novel target for anti-malarial chemotherapy. Biochem J. 2000, 349: 67-75. 10.1042/0264-6021:3490067.PubMed CentralView ArticlePubMed
- Carter NS, Ben Mamoun C, Liu W, Silva EO, Landfear SM, Goldberg DE, Ullman B: Isolation and functional characterization of the PfNT1 nucleoside transporter gene from Plasmodium falciparum. J Biol Chem. 2000, 275: 10683-10691. 10.1074/jbc.275.14.10683.View ArticlePubMed
- El Bissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Ben Mamoun C: The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum. Proc Natl Acad Sci USA. 2006, 103: 9286-9291. 10.1073/pnas.0602590103.PubMed CentralView ArticlePubMed
- Ontyd J, Schrader J: Measurement of adenosine, inosine, and hypoxanthine in human plasma. J Chromatogr. 1984, 307: 404-409. 10.1016/S0378-4347(00)84113-4.View ArticlePubMed
- Sollevi A: Cardiovascular effects of adenosine in man; possible clinical implications. Prog Neurobiol. 1986, 27: 319-349. 10.1016/0301-0082(86)90005-5.View ArticlePubMed
- Lisk G, Kang M, Cohn JV, Desai SA: Specific inhibition of the plasmodial surface anion channel by dantrolene. Eukaryot Cell. 2006, 5: 1882-1893. 10.1128/EC.00212-06.PubMed CentralView ArticlePubMed
- Gero AM, Hall ST: Plasmodium falciparum: transport of entantiomers of nucleosides into Sendai-treated trophozoites. Exp Parasitol. 1997, 86: 228-231. 10.1006/expr.1997.4176.View ArticlePubMed
- de Koning HP, Bridges DJ, Burchmore RJ: Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy. FEMS Microbiol Rev. 2005, 29: 987-1020. 10.1016/j.femsre.2005.03.004.View ArticlePubMed
- Downie MJ, Saliba KJ, Howitt SM, Broer S, Kirk K: Transport of nucleosides across the Plasmodium falciparum parasite plasma membrane has characteristics of PfENT1. Mol Microbiol. 2006, 60: 738-748. 10.1111/j.1365-2958.2006.05125.x.View ArticlePubMed
- Cassera MB, Hazleton KZ, Riegelhaupt PM, Merino EF, Luo M, Akabas MH, Schramm VL: Erythrocytic adenosine monophosphate as an alternative purine source in Plasmodium falciparum. J Biol Chem. 2008, 283: 32889-32899. 10.1074/jbc.M804497200.PubMed CentralView ArticlePubMed
- Kirk K, Staines HM, Martin RE, Saliba KJ: Transport properties of the host cell membrane. Novartis Found Symp. 1999, 226: 55-66. full_text.PubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.