Malaria and pre-eclampsia in an area with unstable malaria transmission in Central Sudan
© Adam et al; licensee BioMed Central Ltd. 2011
Received: 20 April 2011
Accepted: 7 September 2011
Published: 7 September 2011
Placental malaria and pre-eclampsia occur frequently in women in tropics and are leading causes of maternal and perinatal morbidities and mortality. Few data exist concerning the interaction between placental malaria and pre-eclampsia.
A case control study was conducted in Medani Hospital, which locates in an area of unstable malaria transmission in Central Sudan. Case (N = 143) were women with pre-eclampsia, which was defined as systolic blood presure≥140 mm Hg or diastolic blood pressure ≥ 90 mm Hg and proteinuria. Controls were parturient women (N = 143) without any blood pressure values > 139/89 mm Hg or proteinuria. Obstetrical and medical characteristics were gathered from both groups through structured questionnaires. Placental histopathology examinations for malaria were performed.
Twenty-eight (19.6%) vs. 16 (11.2%); P = 0.04 of the cases vs. controls, had placental malaria infections. Five (2%), 1 (2%) and 22 (28.0%) vs. 1, 2 and 13 of the placentae showed acute, chronic and past infection on histopathology examination in the two groups respectively, while 115 (80.4%) vs.127 (88.8%) of them showed no infection, P = 0.04. In multivariate analysis, while there were no associations between age, parity, educational level, lack of antenatal care, blood groups and body mass index and pre-eclampsia; family history of hypertension and placental malaria (OR = 2.3, 95% CI = 1.0-5.2; P = 0.04) were significantly associated with pre-eclampsia.
Placental malaria was associated with pre-eclampsia. Further research is needed.
Pre-eclampsia, one of the most common medical complications of pregnancy, it affects approximately 10% of all human births . It is a leading cause of maternal mortality worldwide, as well as an important cause of perinatal mortality . It has been estimated that 90% of the global malaria burden occurs in sub-Saharan Africa, where during pregnancy 40% women are exposed to malaria infections . Malaria during pregnancy poses a substantial risk to the mother, her fetus and the neonate . Although pre-eclampsia and maternal malaria would be expected frequently to occur concurrently in malarious areas, their interaction on the health of the mother and her baby has been little studied . Seasonal changes in the incidence of pre-eclampsia have been described in tropics, which are consistent with malaria transmission periods . Placental malaria is likely to impair placental development and cause maternal hypertension and placental vascular dysfunction .
Few data exist concerning placental malaria and its association with pre-eclampsia. While some of these studies have reported the association between malaria and pre-eclampsia and malaria and hypertension during pregnancy [8, 9], other studies failed to find a significant association between malaria and pre-eclampsia [10, 11]. Furthermore some of these studies did not differentiate between pre-eclampsia and other types of hypertension.
Malaria during pregnancy is a major health problem in Sudan, where pregnant Sudanese women are more susceptible to malaria, and it is associated with maternal anaemia and poor maternal and perinatal outcomes [12–14]. Both malaria and pre-eclampsia were among the common causes of high maternal mortality in Sudan [15, 16]. The current study was conducted in Wad Medani maternity hospital in central Sudan to investigate the association between placental malaria and pre-eclampsia, so as to add on the standing and the recent research on both placental malaria and pre-eclampsia in Sudan [17, 18]. The area is characterized by unstable malaria transmission and Plasmodium falciparum is the sole malaria species in the area .
A case control study was conducted during the period of June 2009 through January 2010, at the labour ward of Medani Maternity Hospital, Central Sudan. Medani Maternity Hospital has a tertiary care for women who receive antenatal care at the hospital as well as for referrals from the other clinics and hospitals, and women who are close to the hospital facility. All women with risk factors or obstetric complications are referred to the hospital. The referral criteria are not strictly adhered to and many patients without any significant complications deliver at the hospital.
A case was defined as a woman who had given birth and who, in the antenatal period or before going into labour, was diagnosed as being pre-eclamptic. Pre-eclampsia was defined as pregnancy -induced hypertension associated with proteinuria. Pregnancy-induced hypertension was defined as new hypertension with blood pressure of 140 mm Hg systolic or diastolic blood pressure of 90 mm Hg diastolic or greater arising after 20 weeks of gestation in a woman who was normotensive before 20 weeks gestation. Proteinuria was defined as excretion of 300 mg or more of protein in 24 hour urine sample or ≥ 2+ or more on dipstick. A consecutive control was taken for each case. Controls were parturient women admitted for delivery, without any blood pressure values greater than 139/89 or proteinuria recorded in the pregnant health card during the antenatal visits and at the time of delivery. Two measures of blood pressure were done using a sphygmomanometer. Blood pressure measures were taken at the time of admission after a 10-minute rest and far from a uterine contraction; the second measure of blood pressure was taken between 30 and 60 minutes later in the same conditions. Proteinuria (albumin) was detected in a fresh urine sample before active labor by dipsticks (Albustix). Twins and diabetic women were excluded because these were known predictors for pre-eclampsia . After signing an informed consent, from women in both case and control groups, socio-demographic, obstetrics and medical characteristics were gathered through structured questionnaires. Women were enquired for using bed nets and malaria infections in the index pregnancy. Body mass index was calculated by measuring maternal weight and height and expressed as weight (kg)/height (m) 2).
Maternal, placental and cord blood films were prepared, the slides were Giemsa-stained and the number of asexual P. falciparum parasites per 200 white blood cells was counted and double-checked blindly by an expert microscopist. Maternal blood groups were investigated by the agglutination method.
Full thickness placental blocks of around 2-3 cm were taken from the placenta, kept in neutral buffer formalin for histopathology examinations. Placental malaria infections were characterized based on the classification of Bulmer et al : uninfected (no parasites or pigment), acute (parasites in intervillous spaces), chronic (parasites in maternal erythrocytes and pigment in fibrin or cells within fibrin and/or chorionic villous syncytiotrophoblast or stroma), past (no parasites and pigment confined to fibrin or cells within fibrin).
Data were entered in computer using SPSS for windows version 16.0. Means and proportions were compared by Students' t-test, × 2 and Fisher's exact tests as appropriate. Univariate and multivariate analyses were performed where pre-eclampsia as a dependent variable and maternal socio-demographic characteristics (age, parity and maternal blood group, past history and family history of pre-eclampsia) and placental malaria as possible influencing factors. P ≤ 0.05 was regarded as significant.
The study received ethical clearance from the Research Board at the Faculty of Medicine, University of Khartoum.
Socio-demographic and malaria state in the cases and controls
Number (%) of
Pre-eclamptic (N= 143)
lack of antenatal care
Educational level < secondary
History of malaria
Family history of hypertension
Maternal and placental blood films for malaria were positive in one of the cases and one of the controls had positive placental blood film. Twenty-eight (19.6%) vs. 16 (11.2%); P = 0.04 of the cases vs. controls, had placental malaria infections. Five (2%), 1 (2%) and 22 (28.0%) vs. 1, 2 and 13 of the placentae showed acute, chronic and past infection on histopathology examination in the two groups respectively, while 115 (80.4%) vs.127 (88.8%) of them showed no infection, P = 0.04.
Predictors for pre-eclampsia in univariate and multivariate analysis
Factors associated with pre-eclampsia in Medani Maternity hospital, Central Sudan using univariate or multivariate analysis
Family history of hypertension
History of malaria
Educational level < secondary
Lack of antenatal care
Body mass index
Blood group O vs.non group O
Primigravidae, lack of antenatal care and history of malaria in the index pregnancy were associated with pre-eclampsia in univariate analysis only. In multivariate analysis family history of hypertension (OR = 5.7, 95% CI = 2.9-11.5; P = 0.001) and placental malaria (OR = 2.3, 95% CI = 1.0-5.2; P = 0.04) were significantly associated with pre-eclampsia, Table 2.
The main findings of the current study were; there was no significant association between age, educational level, body mass index and blood group and pre-eclampsia. While women with family history of hypertension and those who had placental malaria infections were at higher risk of pre-eclampsia in multivariate analyses, primigravidae, lack of antenatal care and history of malaria in the index pregnancy were associated with pre-eclampsia in univariate analysis only. Recently, age, parity, body mass index were the risk factors for pre-eclampsia observed among Latin American and Caribbean women .
In the current study women who had placental malaria infections were twice at higher risk for pre-eclampsia (OR = 2.3, 95% CI = 1.0-5.2; P = 0.04) compared with women without pre-eclampsia. This is consistent with the previous observation in Senegal with a 3.3-fold increase in the risk of placental malaria infections within women with diastolic blood pressure greater than or equal to 90 mm Hg compared with controls, where it was less than 90 mm Hg . The difference between the current study and the later one is the classification of hypertensive women according to the presence or absence of significant proteinuria in the current study. Pre-eclampsia has also been shown to be more common in Senegal during the rainy season . Likewise, in Tanzania, young first-time mothers with placental malaria had significantly increased risk of hypertension . Interestingly, recently Ndao et al  observed association between placental malaria infections (using placental histology as diagnostic tool) and non-proteinuric hypertension in women living in a malaria-hypoendemic area in Senegal. Yet they did not find an association between placental malaria and pre-eclampsia . However in neighbouring Kenya studies failed to find a relationship between malaria during pregnancy and pre-eclampsia [10, 11].
The majority of the placental infections in the current study were past infections.
There is a critical phase in early/mid pregnancy when transformation of the spiral arteries should take place, which is critical in pathogenesis of future pre-eclampsia.
Massive sequestration of parasites in the placenta leads to placental ischemia and loss of placental integrity, increases production of pro-inflammatory cytokines, and increases endothelial dysfunction [24, 25]. Moreover, it has recently been observed that the production/imbalance of cytokines as pathophysiologic mechanisms of pre-eclampsia and placental malaria infections in two different studies [17, 18]. In 2006 in Tanzania, Muehlenbachs et al  have suggested that maternal-foetal conflict involving the inflammatory mediator, vascular endothelial growth factor pathway occurs during placental malaria and that its inhibitor, soluble vascular endothelial growth factor receptor 1 may be involved in a possible relation between chronic placental malaria infections and hypertension in primigravidae. However, it might still not be clear which the cause of effect is.
Family history of hypertension and placental malaria were associated with pre-eclampsia. Larger longitudinal studies are needed.
We wish to thank all the patients for their excellent cooperation and we are very grateful to the local health authority in Geizera State and to the entire staff of Medani Maternity Hospital. This work has been funded by National Fund for promotion of Medical Service, Khartoum, Sudan.
- Robilland PY, Hulsey TC, Dekker GA, Chaouat G: Preeclampsia and human reproduction: an essay of long-term reflection. J Reprod Immunol. 2003, 59: 93-100. 10.1016/S0165-0378(03)00040-8.View ArticleGoogle Scholar
- Roberts JM, Pearsons G, Cutler J, Lindheimer M: Summary of the NHLBI Working Group on research on hypertension during pregnancy. Hypertension. 2003, 41: 437-45. 10.1161/01.HYP.0000054981.03589.E9.View ArticlePubMedGoogle Scholar
- Steketee RW, Nahlen BL, Parise MF, Menendez C: The burden of malaria in pregnancy in malaria-endemic areas. Am J Trop Med Hyg. 2001, 64 (Suppl): 28-35.PubMedGoogle Scholar
- Cot M, Deloron P: Malaria during pregnancy: consequences and interventional perspectives. Med Trop. 2003, 63: 369-380.Google Scholar
- World Health Organization: The Hypertensive Disorders of Pregnancy. 1987, Technical Report Series, No 758, GenevaGoogle Scholar
- Wacker J, Schulz M, Fruhauf J, Chiwora FM, Solomayer E, Bastert G: Seasonal change in the incidence of pre-eclampsia in Zimbabwe. Acta Obstet Gynecol Scand. 1998, 77: 712-716. 10.1080/j.1600-0412.1998.770703.x.View ArticlePubMedGoogle Scholar
- Brabin BJ, Johnson PM: Placental malaria and pre-clampsia through the looking glass backwards?. J Reprod Immunol. 2005, 65: 1-15. 10.1016/j.jri.2004.09.006.View ArticlePubMedGoogle Scholar
- Sartelet H, Rogier C, Milko-Sartelet I, Angel G, Michel G: Malaria-associated pre-eclampsia in Senegal. Lancet. 1996, 347: 1121-View ArticlePubMedGoogle Scholar
- Ndao CT, Dumont A, Fievet N, Doucoure S, Gaye A, Lehesran JY: Placental malarial infection as a risk factor for hypertensive disorders during pregnancy in Africa: a case-control study in an urban area of Senegal, West Africa. Am J Epidemiol. 2009, 170: 847-853. 10.1093/aje/kwp207.View ArticlePubMedGoogle Scholar
- Shulman CE, Marshall T, Dorman EK, Bulmer JN, Cutts F, Peshu N, Marsh K: Malaria in pregnancy: adverse effects on haemoglobin levels and birthweight in primigravidae and multigravidae. Trop Med Int Health. 2001, 6: 770-778. 10.1046/j.1365-3156.2001.00786.x.View ArticlePubMedGoogle Scholar
- Dorman EK, Shulman CE, Kingdom J, Bulmer JN, Mwendwa J, Peshu N, Marsh K: Impaired uteroplacental blood flow in pregnancies complicated by falciparum malaria. Ultrasound Obs Gynecol. 2002, 19: 165-170. 10.1046/j.0960-7692.2001.00545.x.View ArticleGoogle Scholar
- Adam I, Khamis AH, Elbashir MI: Prevalence and risk factors for anaemia in pregnant women of eastern Sudan. Trans R Soc Trop Med Hyg. 2005, 90: 739-743.View ArticleGoogle Scholar
- Adam I, Elhassan EM, Haggaz AE, Ali AA, Adam GK: A perspective of the epidemiology of malaria and anaemia and their impact on maternal and perinatal outcomes in Sudan. J Infect Dev Ctries. 2011, 5: 83-87.View ArticlePubMedGoogle Scholar
- Bader E, Alhaj AM, Hussan AA, Adam I: Malaria and stillbirth in Omdurman Maternity Hospital, Sudan. Int J Gynaecol Obstet. 2010, 109: 144-146. 10.1016/j.ijgo.2009.11.022.View ArticlePubMedGoogle Scholar
- Ali AA, Adam I: Lack of antenatal care, education, and high maternal mortality in Kassala hospital, eastern Sudan during 2005-2009. J Matern Fetal Neonatal Med. 2011, 24: 1077-1078.View ArticlePubMedGoogle Scholar
- Elhassan EM, Mirghani OA, Adam I: High maternal mortality and stillbirth in the Wad Medani Hospital, Central Sudan, 2003-2007. Trop Doct. 2009, 39: 238-239. 10.1258/td.2009.090005.View ArticlePubMedGoogle Scholar
- Bakheit KH, Bayoumi NK, Eltom AM, Elbashir MI, Adam I: Cytokines profiles in Sudanese women with preeclampsia. Hypertens Pregnancy. 2009, 28: 224-229. 10.1080/10641950802601245.View ArticlePubMedGoogle Scholar
- Bayoumi NK, Bakheit KH, Mohmmed AA, Eltom AM, Elbashir MI, Mavoungou E, Adam I: Cytokine profiles in peripheral, placental and cord blood in an area of unstable malaria transmission in eastern Sudan. J Trop Pediatr. 2009, 55: 233-237. 10.1093/tropej/fmn062.View ArticlePubMedGoogle Scholar
- Malik EM, Atta HY, Weis M, Lang A, Puta C, Lettenmaier C: Sudan Roll Back malaria consultative mission: essential actions to support the attainment of the Abuja targets. Sudan RBM Country Consultative Mission Final Report. 2004, Geneva: Roll Back Malaria Partnership 2004Google Scholar
- Conde-Agudelo A, Belizán JM: Risk factors for pre-eclampsia in a large cohort of Latin American and Caribbean women. BJOG. 2000, 107: 75-83. 10.1111/j.1471-0528.2000.tb11582.x.View ArticlePubMedGoogle Scholar
- Bulmer JN, Rasheed FN, Francis N, Morrison L, Greenwood BM: Placental malaria. I. Pathological classification. Histopathology. 1993, 22: 211-218. 10.1111/j.1365-2559.1993.tb00110.x.View ArticlePubMedGoogle Scholar
- Etard JF, Kodio B, Ronsmans C: Seasonal variation in direct obstetric mortality in rural Senegal: role of malaria?. Am J Trop Med Hyg. 2003, 68: 503-504.PubMedGoogle Scholar
- Muehlenbachs A, Mutabingwa TK, Edmonds S, Fried M, Duffy PE: Hypertension and maternal-fetal conflict during placental malaria. PLoS Med. 2006, 3: e446-10.1371/journal.pmed.0030446.PubMed CentralView ArticlePubMedGoogle Scholar
- Dorman E, Shulman C: Malaria in pregnancy. Mini-symposium: infections in pregnancy. Curr Obstet Gynaecol. 2000, 10: 183-189. 10.1054/cuog.2000.0129.View ArticleGoogle Scholar
- Challier JC, Uzan S: Le placenta humain et ses pathologies: l'oxygène en question. Médecine Sciences. 2003, 19: 1111-1120.View ArticlePubMedGoogle Scholar
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